人MMP9抗体对试剂盒(ab213741)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 15.6 pg/ml - 1000 pg/ml
概述
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产品名称
人MMP9抗体对试剂盒
参阅全部 MMP9 试剂盒 -
检测方法
Colorimetric -
检测类型
ELISA set -
灵敏度
3.77 pg/ml -
范围
15.6 pg/ml - 1000 pg/ml -
种属反应性
与反应: Human -
产品概述
Human MMP9 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human MMP9.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 5 x 96 tests Human MMP9 Capture Antibody 1 x 50µg Human MMP9 Detector Antibody 1 x 12.5µg Human MMP9 Lyophilized Protein 1 vial -
研究领域
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功能
May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. -
组织特异性
Produced by normal alveolar macrophages and granulocytes. -
疾病相关
Intervertebral disc disease
Metaphyseal anadysplasia 2 -
序列相似性
Belongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin repeats. -
结构域
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻译后修饰
Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
N- and O-glycosylated. -
细胞定位
Secreted, extracellular space, extracellular matrix. - Information by UniProt
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别名
- 82 kDa matrix metalloproteinase-9
- 92 kDa gelatinase
- 92 kDa type IV collagenase
see all -
数据库链接
- Entrez Gene: 4318 Human
- Omim: 120361 Human
- SwissProt: P14780 Human
- Unigene: 297413 Human
相关产品
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Alternative Versions
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Related Products
图片
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Standard calibration curve. Background subtracted values are graphed. Data provided for demonstration purposes only.
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To learn more about the advantages of recombinant antibodies see here.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab213741 尚未被引用在任何文献中。