人MIP5 ELISA试剂盒(CCL15) (ab100598)
Key features and details
- Sensitivity: 3 pg/ml
- Range: 4.1 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
-
产品名称
人MIP5 ELISA试剂盒(CCL15)
参阅全部 Macrophage inflammatory protein 5 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 3 pg/ml -
范围
4.1 pg/ml - 1000 pg/ml -
回收率
100 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 101.5 91% - 112% Serum 105.3 92% - 115% Plasma 98.23 90% - 114% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s MIP5 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIP5 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human MIP5 coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIP5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
说明
Optimization may be required with urine samples.
-
平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human MIP5 2 vials MIP5 Microplate (12 strips x 8 wells) 1 unit Recombinant Human MIP5 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
-
功能
Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the small-inducible cytokine A15. -
组织特异性
Most abundant in heart, skeletal muscle and adrenal gland. Lower levels in placenta, liver, pancreas and bone marrow. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are found in high levels in synovial fluids from rheumatoid patients. -
序列相似性
Belongs to the intercrine beta (chemokine CC) family. -
细胞定位
Secreted. - Information by UniProt
-
别名
- C-C motif chemokine 15
- C-C motif chemokine ligand 15
- CC chemokine 3
see all -
数据库链接
- Entrez Gene: 6359 Human
- Omim: 601393 Human
- SwissProt: Q16663 Human
- Unigene: 272493 Human
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab100598 被引用在 1 文献中.
- Pang N et al. Endothelial cell-derived CCL15 mediates the transmigration of fibrocytes through the CCL15-CCR1 axis in vitro. Mol Med Rep 22:5339-5347 (2020). PubMed: 33174007