Key features and details
- Sensitivity: 2.5 pg/ml
- Range: 4.1 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
产品名称人MIP1 beta ELISA试剂盒(CCL4)
参阅全部 CCL4/MIP-1 beta 试剂盒
样品类型Cell culture supernatant, Serum, Plasma
灵敏度< 2.5 pg/ml
范围4.1 pg/ml - 1000 pg/ml
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 97.42 85% - 104% Serum 95.64 82% - 102% Plasma 94.32 83% - 103%
实验步骤Multiple steps standard assay
Abcam’s MIP1 beta Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIP1 beta in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human MIP1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman MIP-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
This product may also recognise CC4L [C-C motif chemokine 4-like], a family member which shares high homology with CCL4.
存放说明Store at -20°C. Please refer to protocols.
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human MIP1 beta 2 vials MIP1 beta Microplate (12 x 8 wells) 1 unit Recombinant Human MIP1 beta Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
功能Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.
序列相似性Belongs to the intercrine beta (chemokine CC) family.
翻译后修饰N-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes.
- Information by UniProt
- MIP 1 beta
- Secreted protein G 26
- ACT 2
ab100597 被引用在 2 文献中.
- Hosseinkhani B et al. (Sub)populations of extracellular vesicles released by TNF-a -triggered human endothelial cells promote vascular inflammation and monocyte migration. J Extracell Vesicles 9:1801153 (2020). PubMed: 32944190
- Hosseinkhani B et al. Extracellular Vesicles Work as a Functional Inflammatory Mediator Between Vascular Endothelial Cells and Immune Cells. Front Immunol 9:1789 (2018). PubMed: 30131806