人MIP-3 alpha ELISA试剂盒(CCL20) (ab269562)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 5.11 pg/ml
- Range: 18.75 pg/ml - 1200 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人MIP-3 alpha ELISA试剂盒(CCL20)
参阅全部 Macrophage Inflammatory Protein 3 alpha 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Supernatant 8 3% 批次间 样品 n Mean SD CV% Supernatant 3 3.1% -
样品类型
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
5.11 pg/ml -
范围
18.75 pg/ml - 1200 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 92 84% - 104% Serum 86 83% - 90% Hep Plasma 98 88% - 106% EDTA Plasma 88 83% - 90% Cit plasma 96 84% - 93% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human MIP-3 alpha ELISA Kit (CCL20) (ab269562) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MIP-3 alpha (CCL20) protein in cell culture supernatant, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MIP-3 alpha (CCL20) with 5.11 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
MIP-3 alpha (MIP3a, CCL20) is a CC chemokine with a selective chemotactic activity for lymphocytes and dendritic cells. Its main function is to inhibit proliferation of myeloid progenitors in colony formation assays. MIP-3 alpha may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. MIP-3 alpha is expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. There are also low levels in thymus, prostate, testis, small intestine and colon. MIP-3 alpha is induced by bacterial lipopolysaccharides, TNF and IFNG/IFN-gamma. Stimulation with phorbol myristate acetate (PMA) in U-937 cell line and Bowes melanoma will induce expression. MIP-3 alpha shares 64.2%, 60.8%, 69.8%, 86.5% sequence homology with mouse, rat, cow, and monkey, respectively.
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平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Human MIP-3 alpha (CCL20) Capture Antibody 1 x 600µl 10X Human MIP-3 alpha (CCL20) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human MIP-3 alpha (CCL20) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. -
组织特异性
Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon. -
序列相似性
Belongs to the intercrine beta (chemokine CC) family. -
翻译后修饰
C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
细胞定位
Secreted. - Information by UniProt
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别名
- Beta chemokine exodus 1
- Beta-chemokine exodus-1
- C C motif chemokine ligand 20
see all -
数据库链接
- Entrez Gene: 6364 Human
- Omim: 601960 Human
- SwissProt: P78556 Human
- Unigene: 75498 Human
相关产品
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Alternative Versions
图片
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Standard Curve comparison between the original human MMP3 SimpleStep ELISA kit (ab178015) and current human MMP3 SimpleStep ELISA kit (ab269562).
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The MIP-3 alpha standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of MIP-3 alpha were measured in duplicates, interpolated from the MIP-3 alpha standard curves and corrected for sample dilution. Undiluted samples are as follows: unstimulated PBMC 100%, 1.5% PHA stimulated PBMC cell culture supernatant 50%, unstimulated THP-1 100%, and 1ug/mL IFN-gamma and 1ug/mL LPS stimulated THP-1 cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MIP-3 alpha concentration was determined to be undetectable in unstimulated PBMC supernatant, 1614.14 pg/mL in PHA stimulated PBMC, undetectable in unstimulated THP-1 supernatant, and 1209.37 pg/mL in IFN-gamma and LPS stimulated THP-1 supernatant.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab269562 被引用在 3 文献中.
- Ng CY et al. Endothelial-immune crosstalk contributes to vasculopathy in nonalcoholic fatty liver disease. EMBO Rep 23:e54271 (2022). PubMed: 35403791
- Wang S et al. Role of chemokine-mediated angiogenesis in resistance towards crizotinib and its reversal by anlotinib in EML4-ALK positive NSCLC. J Transl Med 20:248 (2022). PubMed: 35642002
- Ng CY et al. Endothelial-immune crosstalk contributes to vasculopathy in nonalcoholic fatty liver disease. EMBO Rep 23:e55871 (2022). PubMed: 36200589