人LYVE ELISA试剂盒(ab100585)
Key features and details
- Sensitivity: 8 pg/ml
- Range: 8.19 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人LYVE ELISA试剂盒
参阅全部 LYVE1 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 8 pg/ml -
范围
8.19 pg/ml - 2000 pg/ml -
回收率
78 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 76 69% - 85% Serum 76.38 67% - 87% Plasma 79.07 68% - 86% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Human LYVE ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human LYVE in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human LYVE coated on a 96-well plate. Standards and samples are pipetted into the wells and LYVE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human LYVE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LYVE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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说明
Optimization may be required with urine samples.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human LYVE 2 vials LYVE Microplate (12 x 8 wells) 1 unit Recombinant Human LYVE Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes. -
组织特异性
Mainly expressed in endothelial cells lining lymphatic vessels. -
序列相似性
Contains 1 Link domain. -
翻译后修饰
O-glycosylated. -
细胞定位
Membrane. Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface. - Information by UniProt
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别名
- Cell surface retention sequence-binding protein 1
- CRSBP 1
- CRSBP-1
see all -
数据库链接
- Entrez Gene: 10894 Human
- Omim: 605702 Human
- SwissProt: Q9Y5Y7 Human
- Unigene: 655332 Human
图片
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Representative Standard Curve using ab100585.
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Representative Standard Curve using ab100585.
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ab100585 was used to detect LYVE in Human heparin plasma samples according to the kit protocol. 10 µL sample was used per well.
This image is courtesy of an anonymous Abreview
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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