人Lactoferrin ELISA试剂盒(HLF2) (ab108882)
Key features and details
- Sensitivity: 0.17 ng/ml
- Range: 0.313 ng/ml - 20 ng/ml
- Sample type: Cell culture supernatant, Cell Lysate, Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum, Tissue, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Lactoferrin ELISA试剂盒(HLF2)
参阅全部 Lactoferrin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 3.5% 批次间 样品 n Mean SD CV% Overall 9.8% -
样品类型
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Tissue, Cell Lysate, Cerebral Spinal Fluid -
检测类型
Sandwich (quantitative) -
灵敏度
= 0.17 ng/ml -
范围
0.313 ng/ml - 20 ng/ml -
回收率
= 96 %
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检测时间
4h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Lactoferrin (HLF2) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Lactoferrin levels in Human cell culture supernatants, serum, urine, saliva, milk, cerebrospinal fluid and plasma.
A Lactoferrin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Lactoferrin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Lactoferrin captured in plate.
Get results in 90 minutes with Human Lactoferrin ELISA Kit (ab200015) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Lactoferrin Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Lactoferrin Microplate (12 x 8 well strips) 1 unit Lactoferrin Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
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功能
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.
Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration.
Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors.
The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity. -
序列相似性
Belongs to the transferrin family.
Contains 2 transferrin-like domains. -
细胞定位
Secreted. - Information by UniProt
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别名
- GIG12
- Growth inhibiting protein 12
- HLF2
see all -
数据库链接
- Entrez Gene: 4057 Human
- Omim: 150210 Human
- SwissProt: P02788 Human
- Unigene: 529517 Human
相关产品
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SimpleStep ELISA kits
图片
数据表及文件
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SDS download
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Datasheet download
文献 (18)
ab108882 被引用在 18 文献中.
- Sun X et al. Myeloid-Specific Pyruvate-Kinase-Type-M2-Deficient Mice Are Resistant to Acute Lung Injury. Biomedicines 10:N/A (2022). PubMed: 35625931
- Li R et al. What is the performance of novel synovial biomarkers for detecting periprosthetic joint infection in the presence of inflammatory joint disease? Bone Joint J 103-B:32-38 (2021). PubMed: 33380185
- Jung JY et al. Salivary Immunoglobulin Gamma-3 Chain C Is a Promising Noninvasive Biomarker for Systemic Lupus Erythematosus. Int J Mol Sci 22:N/A (2021). PubMed: 33573068
- Reyes L et al. -------A type I IFN, prothrombotic hyperinflammatory neutrophil signature is distinct for COVID-19 ARDS--. Wellcome Open Res 6:38 (2021). PubMed: 33997298
- Ozuna H et al. The Hunger Games: Aggregatibacter actinomycetemcomitans Exploits Human Neutrophils As an Epinephrine Source for Survival. Front Immunol 12:707096 (2021). PubMed: 34456916