人Interferon gamma ELISA试剂盒(IFNG) (ab100537)
Key features and details
- Sensitivity: 15 pg/ml
- Range: 20.6 pg/ml - 15000 pg/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Interferon gamma ELISA试剂盒(IFNG)
参阅全部 Interferon gamma 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 15 pg/ml -
范围
20.6 pg/ml - 15000 pg/ml -
回收率
> 87 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 94.53 84% - 104% Plasma 86.82 81% - 102% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Interferon gamma (IFNG) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Interferon gamma- in plasma, and cell culture supernatants. (Human Interferon gamma concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human Interferon gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and Interferon gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Interferon gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Interferon gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Human Interferon gamma in normal serum with ab100537, only in serum spiked with Human Interferon gamma.
Get results in 90 minutes with Human IFN gamma ELISA Kit (ab174443) from our SimpleStep ELISA® range.
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说明
Optimization may be required with urine samples
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human Interferon gamma 2 vials Interferon gamma Microplate (12 x 8 wells) 1 unit Recombinant Human Interferon gamma Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. -
组织特异性
Released primarily from activated T lymphocytes. -
疾病相关
In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis. -
序列相似性
Belongs to the type II (or gamma) interferon family. -
翻译后修饰
Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. -
细胞定位
Secreted. - Information by UniProt
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别名
- IF 1
- IFG
- IFI
see all -
数据库链接
- Entrez Gene: 3458 Human
- Omim: 147570 Human
- SwissProt: P01579 Human
- Unigene: 856 Human
相关产品
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SimpleStep ELISA kits
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab100537 被引用在 3 文献中.
- Zan J et al. Paraspeckle Promotes Hepatocellular Carcinoma Immune Escape by Sequestering IFNGR1 mRNA. Cell Mol Gastroenterol Hepatol 12:465-487 (2021). PubMed: 33667716
- Sasawatari S et al. Blockade of N-Glycosylation Promotes Antitumor Immune Response of T Cells. J Immunol 204:1373-1385 (2020). PubMed: 31969386
- Hosseinzadeh F et al. Natural Killer Cell Expansion with Autologous Feeder Layer and Anti-CD3 Antibody for Immune Cell Therapy of Hepatocellular Carcinoma. Asian Pac J Cancer Prev 20:3797-3803 (2019). PubMed: 31870124