人Interferon Alpha 2 ELISA试剂盒(ab233622)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 56.04 pg/ml
- Range: 187.5 pg/ml - 12000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Interferon Alpha 2 ELISA试剂盒
参阅全部 Interferon alpha 2 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Spiked serum 3 2.7% 批次间 样品 n Mean SD CV% Spiked serum 8 9.3% -
样品类型
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
56.04 pg/ml -
范围
187.5 pg/ml - 12000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 103 99% - 109% Cell culture media 106 100% - 116% Hep Plasma 104 94% - 108% EDTA Plasma 87 80% - 93% Cit plasma 103 102% - 103% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human Interferon Alpha 2 ELISA Kit (ab233622) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Interferon Alpha 2 protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Interferon Alpha 2 with 56.04 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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平台
Microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10 x 96 tests 10X Human Interferon Alpha 2 Capture Antibody 1 x 600µl 1 x 6000µl 10X Human Interferon Alpha 2 Detector Antibody 1 x 600µl 1 x 6000µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 200ml Antibody Diluent 5BI 1 x 6ml 10 x 6ml Human Interferon Alpha 2 Lyophilized Purified Protein 2 vials 2 x 10 vials Plate Seals 1 unit 1 x 10 units Sample Diluent NS (ab193972) 1 x 50ml 2 x 250ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 1 x 10 units Stop Solution 1 x 12ml 1 x 120ml TMB Development Solution 1 x 12ml 1 x 120ml -
研究领域
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功能
Produced by macrophages, IFN-alpha have antiviral activities. -
序列相似性
Belongs to the alpha/beta interferon family. -
细胞定位
Secreted. - Information by UniProt
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别名
- Alpha 2a interferon
- IFN
- IFN alpha 2
see all -
数据库链接
- Entrez Gene: 3440 Human
- Omim: 147562 Human
- SwissProt: P01563 Human
- Unigene: 211575 Human
相关产品
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Alternative Versions
图片
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Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of Interferon Alpha 2 were measured in duplicates, interpolated from the Interferon Alpha 2 standard curves.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
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