人Insulin like Growth Factor 1 ELISA试剂盒(IGF1) (ab108873)
Key features and details
- Sensitivity: 1.2 ng/ml
- Range: 0.4 ng/ml - 24 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Insulin like Growth Factor 1 ELISA试剂盒(IGF1)
参阅全部 IGF1 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall 6% 批次间 样品 n Mean SD CV% Overall 10.6% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
> 1.2 ng/ml -
范围
0.4 ng/ml - 24 ng/ml -
回收率
= 98 %
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检测时间
5h 00m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Insulin like Growth Factor 1 (IGF1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Insulin like Growth Factor 1 concentrations in Human cell culture supernatants, serum and plasma.
An Insulin like Growth Factor 1 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Insulin like Growth Factor 1 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Insulin like Growth Factor 1 captured in plate.
Get results in 90 minutes with Human IGF1 ELISA Kit (ab211651) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1X Plasma/Serum Pretreatment Buffer 1 x 7.5ml 1X Standard Diluent 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Insulin like Growth Factor 1 Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Insulin like Growth Factor 1 Microplate (12 x 8 well strips) 1 unit Insulin like Growth Factor 1 Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究领域
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功能
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. -
疾病相关
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. -
序列相似性
Belongs to the insulin family. -
细胞定位
Secreted. - Information by UniProt
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别名
- IBP1
- IGF I
- IGF IA
see all -
数据库链接
- Entrez Gene: 3479 Human
- Omim: 147440 Human
- SwissProt: P05019 Human
- SwissProt: P01343 Human
- Unigene: 160562 Human
相关产品
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SimpleStep ELISA kits
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab108873 被引用在 5 文献中.
- Dammermann W et al. Insulin-like growth factor 1 and dehydroepiandrosterone levels in alcoholic liver cirrhosis. JGH Open 6:723-726 (2022). PubMed: 36262536
- Andrade VFC et al. Bone Histomorphometry in Young Patients With Type 2 Diabetes is Affected by Disease Control and Chronic Complications. J Clin Endocrinol Metab 105:N/A (2020). PubMed: 31587051
- Arablou T et al. Association between dietary intake of some antioxidant micronutrients with some inflammatory and antioxidant markers in active Rheumatoid Arthritis patients. Int J Vitam Nutr Res N/A:1-8 (2019). PubMed: 30932790
- Zhang Y et al. A Novel Approach via Surface Modification of Degradable Polymers With Adhesive DOPA-IGF-1 for Neural Tissue Engineering. J Pharm Sci 108:551-562 (2019). PubMed: 30321547
- Huang G et al. Platelet-rich plasma shows beneficial effects for patients with knee osteoarthritis by suppressing inflammatory factors. Exp Ther Med 15:3096-3102 (2018). PubMed: 29599843