人IL-9 ELISA试剂盒(ab100577)
Key features and details
- Sensitivity: 0.1 ng/ml
- Range: 0.04 ng/ml - 30 ng/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人IL-9 ELISA试剂盒
参阅全部 IL-9 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 0.1 ng/ml -
范围
0.04 ng/ml - 30 ng/ml -
回收率
98 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 102.1 90% - 110% Plasma 82.4 67% - 98% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s IL-9 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-9 in plasma and cell culture supernatants. (Human IL-9 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human IL-9 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Human IL-9 in normal serum with ab100577, only in serum spiked with Human IL-9.
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说明
Optimization may be required with urine samples.
The standard is affinity purified.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human IL-9 2 vials IL-9 Microplate (12 x 8 wells) 1 unit Recombinant Human IL-9 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Supports IL-2 independent and IL-4 independent growth of helper T-cells. -
序列相似性
Belongs to the IL-7/IL-9 family. -
细胞定位
Secreted. - Information by UniProt
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别名
- Cytokine P40
- Homolog of mouse T cell and mast cell growth factor 40
- HP40
see all -
数据库链接
- Entrez Gene: 3578 Human
- Omim: 146931 Human
- SwissProt: P15248 Human
- Unigene: 960 Human
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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