人IL-8抗体对试剂盒(ab215402)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 1.9 pg/ml - 125 pg/ml
概述
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产品名称
人IL-8抗体对试剂盒
参阅全部 IL-8 试剂盒 -
检测方法
Colorimetric -
检测类型
ELISA set -
灵敏度
0.16 pg/ml -
范围
1.9 pg/ml - 125 pg/ml -
种属反应性
与反应: Human -
产品概述
Human IL-8 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human IL-8.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab214030. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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经测试应用
适用于: IA, ELISAmore details -
平台
Reagents
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 5 x 96 tests Human IL-8 Capture Antibody 1 x 50µg Human IL-8 Detector Antibody 1 x 12.5µg Human IL-8 Lyophilized Protein 1 vial -
研究领域
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功能
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. -
序列相似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻译后修饰
Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. -
细胞定位
Secreted. - Information by UniProt
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别名
- (Ala-IL-8)77
- (Ser-IL-8)72
- 9E3
see all -
数据库链接
- Entrez Gene: 3576 Human
- Omim: 146930 Human
- SwissProt: P10145 Human
- Unigene: 624 Human
相关产品
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Alternative Versions
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Related Products
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SimpleStep ELISA kits
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab215402于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IA |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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说明 |
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IA
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
图片
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Standard calibration curve. Background subtracted values are graphed.
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To learn more about the advantages of recombinant antibodies see here.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab215402 被引用在 1 文献中.
- Krausz AD et al. A Variable Height Microfluidic Device for Multiplexed Immunoassay Analysis of Traumatic Brain Injury Biomarkers. Biosensors (Basel) 11:N/A (2021). PubMed: 34562910