人IL-12 p70 ELISA试剂盒(ab213791)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 7.8 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人IL-12 p70 ELISA试剂盒
参阅全部 IL-12 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% 1 16 18pg/ml 1.31 7.3% 2 16 51pg/ml 3.41 6.7% 3 16 294pg/ml 17.38 5.2% 批次间 样品 n Mean SD CV% 1 24 19pg/ml 1.67 8.8% 2 24 55pg/ml 3.79 6.9% 3 24 310pg/ml 21.39 6.9% -
样品类型
Cell culture supernatant, Serum -
检测类型
Sandwich (quantitative) -
灵敏度
< 2 pg/ml -
范围
7.8 pg/ml - 500 pg/ml -
检测时间
3h 30m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
The Human IL-12(p70) Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213791) is designed for the quantitative detection of Human IL-12(p70) in cell culture supernatants and serum.
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for IL-12(p70) has been pre-coated onto 96-well plates. Standards (Expression system for standard: sf21; Immunogen sequence: (I23-S328)+(R23-S219)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-12(p70) is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-12(p70) amount of sample captured in plate. The standard product used in this kit is recombinant human IL-12(p70) with the molecular mass of 75 kDa.
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说明
Interleukin (IL)-12 is a 70 kDa cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response. The IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT-18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12(p70).
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平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml 1 x 12ml Adhesive Plate Seal 4 units 4 units Antibody Diluent Buffer Green Cap 1 x 12ml 1 x 12ml Anti-Human IL-12(p70) coated Microplate (12 x 8 wells) 1 unit 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl 1 x 100µl Biotinylated anti- Human IL-12(p70) antibody 1 x 100µl 1 x 100µl Lyophilized recombinant Human IL-12(p70) standard 2 vials 2 vials Sample Diluent Buffer Green Cap 1 x 30ml 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml 1 x 10ml Wash Buffer (25X) 1 x 20ml 1 x 20ml -
研究领域
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功能
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. -
序列相似性
Belongs to the IL-6 superfamily. -
细胞定位
Secreted. - Information by UniProt
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别名
- CLMF
- CLMF p35
- CLMF p40
see all -
数据库链接
- Entrez Gene: 3592 Human
- Entrez Gene: 3593 Human
- Omim: 161561 Human
- Omim: 161560 Human
- SwissProt: P29459 Human
- SwissProt: P29460 Human
- Unigene: 673 Human
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab213791 尚未被引用在任何文献中。