Key features and details
- Sensitivity: 0.05 ng/ml
- Range: 0.05 ng/ml - 40 ng/ml
- Sample type: Cell culture supernatant, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
参阅全部 IgG1 试剂盒
样品类型Cell culture supernatant, Saliva, Urine, Serum, Plasma
灵敏度< 0.05 ng/ml
范围0.05 ng/ml - 40 ng/ml
特定样本回收率 样品类型 平均% 范围 Serum 87 75% - 97% Plasma 81.5 71% - 94%
实验步骤Multiple steps standard assay
Abcam’s IgG1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IgG1 in serum and plasma.
This assay employs an antibody specific for Human IgG1 coated on a 96-well plate. Standards and samples are pipetted into the wells and IgG1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IgG1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IgG1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples
存放说明Store at -20°C. Please refer to protocols.
组件 1 x 96 tests 2,000X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 2 x 15ml Biotinylated anti-Human IgG1 2 vials IgG1 Microplate (12 x 8 wells) 1 unit Recombinant Human IgG1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
相关性There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant).
- Ig gamma 1 chain C region
ab100548 被引用在 1 文献中.
- Warkiani ME et al. Membrane-less microfiltration using inertial microfluidics. Sci Rep 5:11018 (2015). PubMed: 26154774