抗人IgG Fab fragment抗体[4A11] (ab771)
Key features and details
- Mouse monoclonal [4A11] to Human IgG Fab fragment
- Suitable for: Flow Cyt, WB
- Reacts with: Cow, Human
- Isotype: IgG2b
概述
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产品名称
抗人IgG Fab fragment抗体[4A11] -
描述
小鼠单克隆抗体[4A11] to人IgG Fab fragment -
宿主
Mouse -
特异性
This antibody reacts with Fab fragment of human IgG. Specificity was confirmed by western blotting analysis of purified Fab fragment, of human IgG, under reducing conditions. -
经测试应用
适用于: Flow Cyt, WBmore details -
种属反应性
与反应: Cow, Human -
免疫原
Full length native protein (purified) (Human).
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阳性对照
- WB: Normal human immunoglobulins. Flow Cyt: Human peripheral blood cells.
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常规说明
This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯化说明
Purified by precipitation and chromatography -
克隆
单克隆 -
克隆编号
4A11 -
骨髓瘤
unknown -
同种型
IgG2b -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
Use a concentration of 1 - 5 µg/ml.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
WB |
Use at an assay dependent concentration.
|
说明 |
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Flow Cyt
Use a concentration of 1 - 5 µg/ml. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. |
靶标
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相关性
Human IgG consists of four subclasses (1-4) that can be recognised by antigen differences in their heavy chains. Each subclass has different biological and physiochemical properties. The IgG subclass may be preferentially produced in response to different antigens. The Fab fragment (fragment antigen binding) is the antigen region of the IgG molecule. It is composed of one constant and one variable domain of each of the heavy and the light chain.
图片
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Flow Cytometry analysis of human peripheral blood cells labeling Human IgG Fab fragment with Anti-Human IgG Fab fragment antibody [4A11] (ab771).
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All lanes : Anti-Human IgG Fab fragment antibody [4A11] (ab771)
Lane 1 : Normal human immunoglobulins
Lane 2 : Normal bovine immunoglobulins
Performed under reducing conditions. -
Surface staining of human peripheral blood cells with ab771. An APC-conjugated goat anti-mouse IgG was used as the secondary antibody.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab771 被引用在 2 文献中.
- Lau YF et al. An adjuvant for the induction of potent, protective humoral responses to an H5N1 influenza virus vaccine with antigen-sparing effect in mice. J Virol 84:8639-49 (2010). ELISA . PubMed: 20538850
- Drexler SK et al. SIGIRR/TIR-8 is an inhibitor of Toll-like receptor signaling in primary human cells and regulates inflammation in models of rheumatoid arthritis. Arthritis Rheum 62:2249-61 (2010). Flow Cyt ; Human . PubMed: 20506350