抗人IgG Fab fragment抗体[4A11] (ab771)
概述
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产品名称
抗人IgG Fab fragment抗体[4A11] -
描述
小鼠单克隆抗体[4A11] to人IgG Fab fragment -
宿主
Mouse -
特异性
This antibody reacts with Fab fragment of human IgG. Specificity was confirmed by western blotting analysis of purified Fab fragment, of human IgG, under reducing conditions. -
经测试应用
适用于: Flow Cyt, ELISA, WBmore details -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified) (Human).
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阳性对照
- WB: Normal human immunoglobulins. Flow Cyt: Human peripheral blood cells.
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常规说明
This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯化说明
Purified by precipitation and chromatography -
克隆
单克隆 -
克隆编号
4A11 -
骨髓瘤
unknown -
同种型
IgG2b -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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Flow Cyt | Use at an assay dependent concentration. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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ELISA | Use at an assay dependent concentration. | |
WB | Use at an assay dependent concentration. |
靶标
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相关性
Human IgG consists of four subclasses (1-4) that can be recognised by antigen differences in their heavy chains. Each subclass has different biological and physiochemical properties. The IgG subclass may be preferentially produced in response to different antigens. The Fab fragment (fragment antigen binding) is the antigen region of the IgG molecule. It is composed of one constant and one variable domain of each of the heavy and the light chain.
图片
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Flow Cytometry analysis of human peripheral blood cells labeling Human IgG Fab fragment with Anti-Human IgG Fab fragment antibody [4A11] (ab771).
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All lanes : Anti-Human IgG Fab fragment antibody [4A11] (ab771)
Lane 1 : Normal human immunoglobulins
Lane 2 : Normal bovine immunoglobulins
Performed under reducing conditions. -
Surface staining of human peripheral blood cells with ab771. An APC-conjugated goat anti-mouse IgG was used as the secondary antibody.
文献
This product has been referenced in:
- Lau YF et al. An adjuvant for the induction of potent, protective humoral responses to an H5N1 influenza virus vaccine with antigen-sparing effect in mice. J Virol 84:8639-49 (2010). ELISA . Read more (PubMed: 20538850) »
- Drexler SK et al. SIGIRR/TIR-8 is an inhibitor of Toll-like receptor signaling in primary human cells and regulates inflammation in models of rheumatoid arthritis. Arthritis Rheum 62:2249-61 (2010). Flow Cyt ; Human . Read more (PubMed: 20506350) »