抗人IgG Fab fragment抗体[4A11] (ab771)

Submit a review Q&A (8)References (2)
Flow Cytometry - Anti-Human IgG Fab fragment antibody [4A11] (ab771)
  • Western blot - Anti-Human IgG Fab fragment antibody [4A11] (ab771)
  • Flow Cytometry - Anti-Human IgG Fab fragment antibody [4A11] (ab771)

Key features and details

  • Mouse monoclonal [4A11] to Human IgG Fab fragment
  • Suitable for: Flow Cyt, WB
  • Reacts with: Cow, Human
  • Isotype: IgG2b

概述

  • 产品名称

    抗人IgG Fab fragment抗体[4A11]

  • 描述

    小鼠单克隆抗体[4A11] to人IgG Fab fragment

  • 宿主

    Mouse

  • 特异性

    This antibody reacts with Fab fragment of human IgG. Specificity was confirmed by western blotting analysis of purified Fab fragment, of human IgG, under reducing conditions.

  • 经测试应用

    适用于: Flow Cyt, WBmore details

  • 种属反应性

    与反应: Cow, Human

  • 免疫原

    Full length native protein (purified) (Human).

  • 阳性对照

    • WB: Normal human immunoglobulins. Flow Cyt: Human peripheral blood cells.

  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 纯化说明

    Purified by precipitation and chromatography

  • 克隆

    单克隆

  • 克隆编号

    4A11

  • 骨髓瘤

    unknown

  • 同种型

    IgG2b

  • 轻链类型

    unknown

  • 研究领域

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
Use a concentration of 1 - 5 µg/ml.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB
Use at an assay dependent concentration.
说明
Flow Cyt
Use a concentration of 1 - 5 µg/ml.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB
Use at an assay dependent concentration.

靶标

  • 相关性

    Human IgG consists of four subclasses (1-4) that can be recognised by antigen differences in their heavy chains. Each subclass has different biological and physiochemical properties. The IgG subclass may be preferentially produced in response to different antigens. The Fab fragment (fragment antigen binding) is the antigen region of the IgG molecule. It is composed of one constant and one variable domain of each of the heavy and the light chain.

图片

  • Flow Cytometry - Anti-Human IgG Fab fragment antibody [4A11] (ab771)
    Flow Cytometry - Anti-Human IgG Fab fragment antibody [4A11] (ab771)
    Flow Cytometry analysis of human peripheral blood cells labeling Human IgG Fab fragment with Anti-Human IgG Fab fragment antibody [4A11] (ab771).
  • Western blot - Anti-Human IgG Fab fragment antibody [4A11] (ab771)
    Western blot - Anti-Human IgG Fab fragment antibody [4A11] (ab771)
    All lanes : Anti-Human IgG Fab fragment antibody [4A11] (ab771)

    Lane 1 : Normal human immunoglobulins
    Lane 2 : Normal bovine immunoglobulins

    Performed under reducing conditions.
  • Flow Cytometry - Anti-Human IgG Fab fragment antibody [4A11] (ab771)
    Flow Cytometry - Anti-Human IgG Fab fragment antibody [4A11] (ab771)

    Surface staining of human peripheral blood cells with ab771. An APC-conjugated goat anti-mouse IgG was used as the secondary antibody.

文献 (2)

发表研究结果有使用 ab771？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab771 被引用在 2 文献中.

  • Lau YF  et al. An adjuvant for the induction of potent, protective humoral responses to an H5N1 influenza virus vaccine with antigen-sparing effect in mice. J Virol 84:8639-49 (2010). ELISA . PubMed: 20538850
  • Drexler SK  et al. SIGIRR/TIR-8 is an inhibitor of Toll-like receptor signaling in primary human cells and regulates inflammation in models of rheumatoid arthritis. Arthritis Rheum 62:2249-61 (2010). Flow Cyt ; Human . PubMed: 20506350

客户评价及客户问答

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1-8 of 8 Abreviews or Q&A

Question

Has product ab771 ever been tested for blocking exposed IgG fab fragment? Is this antibody specific for IgG fab over fc?

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Answer

We have not performed the blocking assay, but the specificity of this antibody for Fab part of IgG (over Fc) was confirmed by Western blotting using Fab fragments.

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Question

Whole human IgG adsorbed to a substrate and am looking to determine the amount of adsorbed protein that has the Fab region exposed (as opposed to the total amount of IgG adsorbed). I was planning on using immunofluorescence (preferably) or ELISA to do this, and was wondering if product ab771 would be suitable for this purpose.

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Answer

This product will recognize the Fab fragment of any exposed IgG. However if whole IgG is present and not masked by absorption into the substrate, these too will be recognized. I would recommend performing a co stain with an anti- IgG Fc specific antibody to determine whether the signal seen by ab771 is from Fabs or whole IgG. We offer a Chicken polyclonal to Human + Mouse + Rat IgG Fc fragment (ab15866) which you would be able to use as a co-stain with ab771. As this product is also against mouse Fc I would recommend sequential staining first with ab15866 followed by ab771.

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Question

We ordered this antibody. Can you provide the affinity constant KD of this antibody against Human Fab?

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Answer

Thank you for ordering the Fab fragment antibody . Unfortunately, the affinity or dissociation constant, Kd, is not determined for this antibody. If you have any other questions, please contact us.

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Question

BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM High background PRIMARY ANTIBODY What is the recommended dilution to use this antibody to detect human IgG on a western blot? SECONDARY ANTIBODY What is the recommended sencondary antibody? DETECTION METHOD ECL Plus

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Answer

Thank you for your enquiry. For Western blotting, I would recommend using ab771 at a dilution of 1:1000 and optimizing based upon results. At the bottom of the online datasheet for ab771 there is a list of compatible secondary antibodies which can be used. One suggestion is ab6728 - Rabbit polyclonal to Mouse IgG H and L (HRP). If you are having problems with high background, you may want to try blocking with 3% BSA, increase the number of washes, and decreasing the concentration of primary antibody as well as the incubation period. Please contact us again if you have any additional questions.

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Question

ANTIBODY CODE ab771 PRE-SALES ENQUIRY Does it recognise IgG4 isoform and does it detect the light or heavy chain on the fab?

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Answer

Thank you for your enquiry. The antibody should recognize all human IgG isoforms. The H or L chain specificity has not been determined. If you need any further assistance please contact us again.

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Question

I want to buy IgG Fab fragment (ab771). But I need to know the isoelectric point? What is also the isoelectric point of the human IgG(ab436)? thank you for your answer,

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Answer

Thank you for your enquiry. The isoelectric point of this antibody has not been determined.

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Question

Customer would like to know if the epitopes are known for ab74987 and ab771.

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Answer

Regarding ab7498, this particular clone is Fc-specific but the epitope has not been more precisely determined. For ab771, the epitope is unknown. If you have any more questions, please contact us again.

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Question

Customer would like to know which would be better to detect human IgG - anti-Fab or anti-Fc?

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Answer

The originator of these two antibodies has informed me that the anti Fab fragment antibody is better (IgG receptors bind IgG via Fc so it may be occupied). The Fc fragment is not good for IgM bound to the B-cell surface, but is great for soluble IgM.

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