人IgG ELISA试剂盒(ab100547)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 0.02 ng/ml - 15 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人IgG ELISA试剂盒
参阅全部 IgG 试剂盒 -
检测方法
Colorimetric -
样品类型
Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 20 pg/ml -
范围
0.02 ng/ml - 15 ng/ml -
回收率
117 %
特定样本回收率 样品类型 平均% 范围 Serum 116.1 105% - 123% Plasma 118.1 99% - 126% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Human IgG ELISA kit is designed for the quantitative measurement of Human IgG in serum and plasma.
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说明
The Human immune system consists of two functional components classified as the innate system (the physical, biochemical and cellular barriers), and the adaptive immune system (including lymphocytes and immunoglobulins). Immunoglobulins are the key elements of the humoral immune response in vertebrate against parasitic invasion.
The polypeptide chains of immunoglobulins composed of two identical heavy (H) chains and two identical light (L) chains linked together by inter-chain disulfide bonds. While the amino-terminal portions that exhibits highly variable amino-acid composition are involved in antigen binding, the C terminal constant parts are involved in complement binding, placental passage and binding to cell membranes. Based upon the variation of the constant region of the heavy chain, nine immunoglobulin heavy chain isotypes are found in humans: IgA (with subclasses IgA1 and IgA2), IgD, IgE, IgM, and IgG (with subclasses IgG1, IgG2, IgG3, and IgG4).
IgG is the predominant immunoglobulin in the serum, which accounts for 75% of the total serum antibody of healthy individuals. IgG has a molecular weight of about 150 kDa. Four distinct subgroups of human IgG (IgG1, IgG2, IgG3, and IgG4) were first demonstrated in the 1960’s by using polyclonal antisera prepared in animals immunized with human myeloma proteins.
The capture antibody of the kit ab100547 detects the Fc portion of the heavy chain of all subclasses of human IgG and the detection antibody detects the F(ab’)2 fragment of IgG.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 4,000X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 2 x 15ml Biotinylated anti-Human IgG 2 vials IgG Microplate (12 x 8 wells) 1 unit Recombinant Human IgG Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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细胞定位
Secreted -
别名
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
see all -
数据库链接
- Entrez Gene: 3500 Human
- Entrez Gene: 3501 Human
- Entrez Gene: 3502 Human
- Entrez Gene: 3503 Human
- SwissProt: P01857 Human
- SwissProt: P01859 Human
- SwissProt: P01860 Human
- SwissProt: P01861 Human
相关产品
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SimpleStep ELISA kits
数据表及文件
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SDS download
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Datasheet download
文献 (19)
ab100547 被引用在 19 文献中.
- Liu Y et al. Sialylation of IgG inhibits the formation of galactose-deficient IgA1-containing immune complexes and protects mesangial cells from injury in IgA nephropathy. BMC Nephrol 23:25 (2022). PubMed: 35016642
- Zhao L et al. Ulinastatin combined with glutamine improves liver function and inflammatory response in patients with severe acute pancreatitis. Am J Transl Res 14:918-926 (2022). PubMed: 35273695
- Wang M et al. A Biomarker Panel Based upon AFP, Fucosylated Kininogen and PEG-Precipitated IgG Is Highly Accurate for the Early Detection Hepatocellular Carcinoma in Patients with Cirrhosis in Phase II and Phase III Biomarker Evaluation. Cancers (Basel) 14:N/A (2022). PubMed: 36497452
- Bachhuber F et al. Diagnostic biomarkers in tear fluid: from sampling to preanalytical processing. Sci Rep 11:10064 (2021). PubMed: 33980989
- Shi X et al. Downregulated miR-29a promotes B cell overactivation by upregulating Crk-like protein in systemic lupus erythematosus. Mol Med Rep 22:841-849 (2020). PubMed: 32467986