概述

  • 产品名称
    抗人IgG抗体[EPR4421]
    参阅全部 Human IgG 一抗
  • 描述
    兔单克隆抗体[EPR4421] to人IgG
  • 宿主
    Rabbit
  • 特异性
    ab109489 recognizes all four Human IgG isotypes (IgG1, IgG2, IgG3, and IgG4).
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide within Human Human IgG aa 150-250 (internal sequence). The exact sequence is proprietary.

  • 阳性对照
    • WB: Human IgG2, IgG1, IgG3, and IgG4 recombinant proteins, human fetal thymus, fetal tonsil and fetal spleen tissue lysates. IHC-P: Human tonsil and stomach tissues.
  • 常规说明

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    单克隆
  • 克隆编号
    EPR4421
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab109489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 52-58 kDa.
IHC-P 1/300 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

靶标

  • 相关性
    IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).
  • 细胞定位
    Cell Membrane and Secreted
  • 数据库链接
  • 别名
    • FLJ39988 antibody
    • FLJ40036 antibody
    • FLJ40253 antibody
    • FLJ40587 antibody
    • FLJ40789 antibody
    • FLJ40834 antibody
    • G1m marker antibody
    • G2m marker antibody
    • G3m marker antibody
    • G4m marker antibody
    • HDC antibody
    • Heavy chain disease protein antibody
    • Human immunglobulin G antibody
    • Ig gamma 1 chain C region antibody
    • Ig gamma 2 chain C region antibody
    • Ig gamma 3 chain C region antibody
    • Ig gamma 4 chain C region antibody
    • IgG heavy chain locus antibody
    • IGHG 1 antibody
    • IGHG 2 antibody
    • IGHG 3 antibody
    • IGHG 4 antibody
    • IGHG1 antibody
    • IGHG2 antibody
    • IGHG3 antibody
    • IGHG4 antibody
    • Immunoglobulin Gm1 antibody
    • Immunoglobulin Gm3 antibody
    • Immunoglobulin Gm4 antibody
    • immunoglobulin heavy constant gamma 1 (G1m marker) antibody
    • Immunoglobulin heavy constant gamma 1 antibody
    • immunoglobulin heavy constant gamma 2 (G2m marker) antibody
    • Immunoglobulin heavy constant gamma 2 antibody
    • immunoglobulin heavy constant gamma 3 (G3m marker) antibody
    • Immunoglobulin heavy constant gamma 3 antibody
    • immunoglobulin heavy constant gamma 4 (G4m marker) antibody
    • Immunoglobulin heavy constant gamma 4 antibody
    • MGC117419 antibody
    • MGC45809 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Human IgG with purified ab109489 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • All lanes : Anti-Human IgG antibody [EPR4421] (ab109489) at 1/1000 dilution (unpurified)

    Lane 1 : IgG2 recombinant protein (10 ng protein loaded)
    Lane 2 : IgG1 recombinant protein (10 ng protein loaded)
    Lane 3 : IgG3 recombinant protein (10 ng protein loaded)
    Lane 4 : IgG4 recombinant protein (10 ng protein loaded)
  • All lanes : Anti-Human IgG antibody [EPR4421] (ab109489) at 1/20000 dilution (purified)

    Lane 1 : Human fetal thymus tissue lysate
    Lane 2 : Human fetal tonsil tissue lysate
    Lane 3 : Human fetal spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling IgG with unpurified ab109489 at a dilution of 1/500.

  • Anti-Human IgG antibody [EPR4421] (ab109489) at 1/1000 dilution (purified) + IgG3 recombinant protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-Human IgG antibody [EPR4421] (ab109489) at 1/5000 dilution (purified)

    Lane 1 : IgG1 recombinant protein
    Lane 2 : IgG2 recombinant protein
    Lane 3 : IgG4 recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling IgG with unpurified ab109489 at a dilution of 1/500.

文献

This product has been referenced in:
  • Sun D  et al. Downregulation of miR-374b-5p promotes chemotherapeutic resistance in pancreatic cancer by upregulating multiple anti-apoptotic proteins. Int J Oncol N/A:N/A (2018). Read more (PubMed: 29568910) »
  • Jing Z  et al. Expression of immunoglobulin G in human podocytes, and its role in cell viability and adhesion. Int J Mol Med 41:3296-3306 (2018). Read more (PubMed: 29512722) »
See all 16 Publications for this product

客户评价及客户问答

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1-3 of 3 Abreviews

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Breast cancer cell line)
Permeabilization
No
Specification
Breast cancer cell line
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

提交于 Jul 05 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Breast carcinoma and Breast cell lines)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6 and EDTA pH8.5
Permeabilization
No
Specification
Breast carcinoma and Breast cell lines
Blocking step
(agent) for 10 minute(s) · Concentration: 50% · Temperature: 24°C
Fixative
Formaldehyde

Mr. Malcolm Lim

Verified customer

提交于 Jun 27 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (PBMC)
Permeabilization
Yes - 4x Cytofix, 45 min, 4 ¯C
Gating Strategy
CD19 positive live cells
Specification
PBMC
Preparation
Cell harvesting/tissue preparation method: PBMC from whole blood by density gradient (lymphoprep, Fresenius)
Sample buffer: D-PBS+5 % FCS + 2 mM EDTA

Clara Lettl

Verified customer

提交于 Jan 18 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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