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Epigenetics and Nuclear Signaling Histones H3 Methylated

人Histone H3 (mono methyl K4)多肽(ab1340)

  • Datasheet
  • SDS
Reviews (1)Q&A (5)References (4)

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Western blot - Human Histone H3 (mono methyl K4) peptide (ab1340)
  • Dot Blot - Human Histone H3 (mono methyl K4) peptide (ab1340)

Key features and details

  • Suitable for: Blocking, Dot blot

描述

  • 产品名称

    人Histone H3 (mono methyl K4)多肽
    参阅全部 Histone H3 蛋白酶
  • 无动物成分

    No
  • 性质

    Synthetic
    • 种属

      Human
    • 修饰

      mono methyl K4

相关产品

  • Corresponding Antibody

    • Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (ab8895)
  • Related Products

    • Anti-Histone H3 (tri methyl K4) antibody [mAbcam1012] - ChIP Grade (ab1012)
    • Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (ab1220)
    • Human Histone H3 (tri methyl K4) peptide (ab1342)
    • Anti-Histone H3 antibody (ab18521)
    • Anti-Histone H3 (di methyl K4, tri methyl K4) antibody [mAbcam 6000] - ChIP Grade (ab6000)
    • Anti-Histone H3 (tri methyl K27) antibody [mAbcam 6002] - ChIP Grade (ab6002)
    • Anti-pan methyl Lysine antibody - ChIP Grade (ab7315)
    • Anti-Histone H3 (di methyl K4) antibody - ChIP Grade (ab7766)
    • Human Histone H3 (di methyl K4) peptide (ab7768)
    • Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (ab8580)

技术指标

Our Abpromise guarantee covers the use of ab1340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Blocking - Blocking peptide for Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (ab8895)

    Dot blot

  • 形式

    Lyophilized
  • 补充说明

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Store at -80°C.

    Information available upon request.

常规信息

  • 别名

    • H3 histone family member E pseudogene
    • H3 histone family, member A
    • H3/A
    • H31_HUMAN
    • H3F3
    • H3FA
    • Hist1h3a
    • HIST1H3B
    • HIST1H3C
    • HIST1H3D
    • HIST1H3E
    • HIST1H3F
    • HIST1H3G
    • HIST1H3H
    • HIST1H3I
    • HIST1H3J
    • HIST3H3
    • histone 1, H3a
    • Histone cluster 1, H3a
    • Histone H3 3 pseudogene
    • Histone H3.1
    • Histone H3/a
    • Histone H3/b
    • Histone H3/c
    • Histone H3/d
    • Histone H3/f
    • Histone H3/h
    • Histone H3/i
    • Histone H3/j
    • Histone H3/k
    • Histone H3/l
    see all
  • 功能

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 序列相似性

    Belongs to the histone H3 family.
  • 发展阶段

    Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
  • 翻译后修饰

    Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
    Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
    Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
    Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
    Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
    Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
  • 细胞定位

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P68431 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

图片

  • Western blot - Human Histone H3 (mono methyl K4) peptide (ab1340)
    Western blot - Human Histone H3 (mono methyl K4) peptide (ab1340)
    All lanes : Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade (ab8895) at 1/500 dilution

    Lane 1 : Calf thymus histone lysate
    Lane 2 : Calf thymus histone lysate with Human Histone H3 (mono methyl K4) peptide (ab1340) at 1 µg/ml
    Lane 3 : Calf thymus histone lysate with Human Histone H3 (di methyl K4) peptide (ab7768) at 1 µg/ml
    Lane 4 : Calf thymus histone lysate with Human Histone H3 (tri methyl K4) peptide (ab1342) at 1 µg/ml
    Lane 5 : Calf thymus histone lysate with Human Histone H3 (mono methyl K9) peptide (ab1771) at 1 µg/ml
    Lane 6 : Calf thymus histone lysate with Human Histone H3 (mono methyl K27) peptide (ab1780) at 1 µg/ml
    Lane 7 : Calf thymus histone lysate with Human Histone H3 (unmodified ) peptide (ab2903) at 1 µg/ml

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution

    Performed under reducing conditions.

    Observed band size: 18 kDa why is the actual band size different from the predicted?


    Exposure time: 2 minutes


    ab8895 is specific for mono-methylated Lysine 4 of histone H3 and does not recognize di- or tri-methyl Lysine 4 nor methylation at Lysine 9. This is shown in lane 2 where the activity of the antibody is specifically blocked by the addition of the immunizing peptide (ab1340).

  • Dot Blot - Human Histone H3 (mono methyl K4) peptide (ab1340)
    Dot Blot - Human Histone H3 (mono methyl K4) peptide (ab1340)This image is courtesy of an anonymous Abreview

    ab1340 (at 0.01 and 0.1 μg) used in Dot Blot to test for cross-reactivity of ab8895 (1/2500). An HRP-conjugated Mouse anti-rabbit IgG polyclonal (1/10000) was used as the secondary antibody.

    See Abreview

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (4)

发表研究结果有使用 ab1340?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab1340 被引用在 4 文献中.

  • You Y  et al. Temporal dynamics of gene expression and histone marks at the Arabidopsis shoot meristem during flowering. Nat Commun 8:15120 (2017). PubMed: 28513600
  • Lin X  et al. Protocol: Chromatin immunoprecipitation (ChIP) methodology to investigate histone modifications in two model diatom species. Plant Methods 8:48 (2012). PubMed: 23217141
  • Wang Y  et al. Regulation of Set9-mediated H4K20 methylation by a PWWP domain protein. Mol Cell 33:428-37 (2009). PubMed: 19250904
  • Mulder KW  et al. Regulation of histone H3K4 tri-methylation and PAF complex recruitment by the Ccr4-Not complex. Nucleic Acids Res 35:2428-39 (2007). PubMed: 17392337

客户评价及客户问答

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提交评价 提交问题

1-6 of 6 Abreviews or Q&A

Question

I am doing research with the model plant Arabidopsis. I can use the antibodies and peptides derived from human, because histone H3 is mostly conserved among many eukaryotes, however there are still differences at some amino acid positions. Therefore the information of the residue sequence or residue positions is very important for me to choose your products.

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Abcam community

Verified customer

Asked on Oct 15 2012

Answer

The sequence and purification method is propriety, but if you let me know what is important for you I can check if one of our product fits.

Read More

Abcam Scientific Support

回复于 Oct 15 2012

Question

Do you have any full size of H3K4 mono-, di- and tri methylated histone 3 which I can load on the gel as positive control.

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Abcam community

Verified customer

Asked on Feb 14 2012

Answer

The molecular weights of the different methylation states be extrememly difficult to distinguish as that type of modification creates a 14Dalton mass change.Currently, we do not offer full length methylated H3K4 proteins. We do have anEpiSeeker Histone Extraction Kit (ab113476) whichprovides a simple and selective method for extracting histone proteins in just 1 hour. When used in conjunction with our peptides you can be confident that the band you are seeing is indeed specific to the mono- di- or tri- methylayed H3K4 protein.

More information about ourEpiSeeker Histone Extraction Kit (ab113476) is available here:

https://www.abcam.com/EpiSeeker-Histone-Extraction-Kit-ab113476.html

Read More

Abcam Scientific Support

回复于 Feb 14 2012

Question

We have your antibodies against Histone H3K4 methylation (ab8895 for monomethylation and ab8580 for trimethylation). I would like to see their specificity on WB. Do you have any WB data loading Histon H3 unmodified, monomethylated H3K4, dimethyated H3K4 and trimethylated H3K4 and then stained by ab8895 or ab8580 ? Also do you have any control peptide/protein applicable for WB of H3K4 methylation WB ? They should be full size of monomethylated H3K4, dimethylated H3K4, trimethylated H3K4 and unmodified H3.

Read More

Abcam community

Verified customer

Asked on Feb 13 2012

Answer

Peptides are available for both of these products. The peptide for ab8895,Anti-Histone H3 (mono methyl K4) antibody - ChIP Grade, is product number ab1340 (https://www.abcam.com/histone-h3-peptide-mono-methyl-k4-ab1340.html).The peptide for ab8580,Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade, is ab1342 (https://www.abcam.com/histone-h3-peptide-tri-methyl-k4-ab1342.html). These are the exact peptides used to create the antibodies. Each peptide used in the testing assay is available as well. (https://www.abcam.com/Human-Histone-H3-mono-methyl-K4-peptide-ab1340.html,https://www.abcam.com/Human-Histone-H3-di-methyl-K4-peptide-ab7768.html),https://www.abcam.com/Human-Histone-H3-tri-methyl-K4-peptide-ab1342.html,https://www.abcam.com/Human-Histone-H3-mono-methyl-K9-peptide-ab1771.html,https://www.abcam.com/Human-Histone-H3-mono-methyl-K27-peptide-ab1780.html,https://www.abcam.com/Human-Histone-H3-unmodified-peptide-ab2903.html.

These products are tested by peptide assay. Our data on western blot is displayed on the datasheet for each product. For ab8895 our test show itspecific for mono-methylated Lysine 4 of histone H3 and does not recognize di- or tri-methyl Lysine 4 nor methylation at Lysine 9. In the image on the datasheet, this is shown in lane 2where the activity of the antibody is specifically blocked by the addition of the immunizing peptide (ab1340) [https://www.abcam.com/Histone-H3-mono-methyl-K4-antibody-ChIP-Grade-ab8895.html#ab8895_6.gif].

Further,ab8580 are tested in Peptide Array against peptides to different Histone H3 modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H3 - tri methyl K4 peptide (ab1342), indicating that this antibody specifically recognises the Histone H3 - tri methyl K4 modification [https://www.abcam.com/Histone-H3-tri-methyl-K4-antibody-ChIP-Grade-ab8580.html#Histone-H3-Primary-antibodies-ab8580-153.jpg.]

Each of these products are thoroughly characterized and published. There are links to specific publications and th Abreviews from our customers on each datasheet.

Read More

Abcam Scientific Support

回复于 Feb 13 2012

Other (Dot Blot) Abreview for Histone H3 peptide - mono methyl K4

Excellent
Abreviews
Abreviews
abreview image
Application
Other - Do not use

Review text: I used this product to do a dot blot specificity test for modified histone antibodies and everything worked great. I made two dilutions of the peptide and spotted it on a nitrocellulose membrane using a Bio-Dot apparatus. Once the peptides were bound to the membrane I incubated with the appropriate primary and secondary antibodies at an assay dependent concentration and developed using ECL.
Sample: Human Purified protein

Primary antibody (in addition to 'Histone H3 peptide - mono methyl K4')
Primary antibody: Abcam primary antibody: Anti-Histone H3 (methylated) antibody (ab8895)
Dilution: 1/2500

Secondary antibody
Name: Non-Abcam antibody was used: anti-rabbit
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/10000

Abcam user community

Verified customer

提交于 Nov 01 2010

Question

One customer has ordered one vial ab1340 from you. the lot No is 17195. the concertration on the label of the vial is 5mg/ml, the according to datasheet on your website, it should be 1mg/ml. so the customer is confused. Please confirm and tell me the correct concertration of this lot ab1340.

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Abcam community

Verified customer

Asked on May 16 2005

Answer

The concentration of lot number: 17195 is 5mg/ml. All subsequence batches of this product are 1mg/ml.

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Abcam Scientific Support

回复于 May 24 2005

Question

But I should be able to use it for mass spectrometry, right? Would you please tell me what is the mass for the peptide?--

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Abcam community

Verified customer

Asked on Oct 25 2004

Answer

Thank you for your enquiry. This product has been tested and characterised for blocking studies, immunofluorescence, peptide competition assay. We regret to inform you that we have not tested it for mass spectrometry so we do not have any information about this type of assay.

Read More

Abcam Scientific Support

回复于 Oct 26 2004

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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