人Hexokinase II ELISA试剂盒(ab219043)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 11.37 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture extracts, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Hexokinase II ELISA试剂盒
参阅全部 Hexokinase II 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% HeLa extract 8 3% 批次间 样品 n Mean SD CV% HeLa extract 3 5.7% -
样品类型
Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
11.37 pg/ml -
范围
62.5 pg/ml - 4000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 99 96% - 102% Cell culture extracts 92 89% - 102% Tissue Extracts 96 91% - 103% Hep Plasma 101 98% - 106% EDTA Plasma 102 97% - 106% Cit plasma 96 91% - 99% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human Hexokinase II ELISA Kit (ab219043) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Hexokinase II protein in cell culture extracts, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Hexokinase II with 11.37 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
Hexokinases are involved in the pathway of hexose metabolism, where they phosphorylate hexose to form hexose-6-phosphate. Phosphorylation of hexose restricts transport out of the cell, therefore retained intracellularly for metabolic processes including glycolysis and glycogen synthesis. Hexokinases also phosphorylate glucose to glucose-6-phosphate.
There are four mammalian hexokinases. Hexokinase II, also known as muscle form hexokinase, localizes to the outer membrane of mitochondria and is present in adipose tissue, skeletal muscle, and heart. Hexokinase II comprises of two homologous halves with both the C-terminal and N-terminal portions being catalytically active (with the N-terminal half having higher activity than the C-terminal half). Human Hexokinase I, mouse Hexokinase II, and rat Hexokinase II share 73.8%, 94.2%, and 94.4% in homology in amino acid sequence with human Hexokinase II, respectively.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Human Hexokinase II Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI2 1 x 6ml Human Hexokinase II Capture Antibody (Lyophilized) 1 vial Human Hexokinase II Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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组织特异性
Predominant hexokinase isozyme expressed in insulin-responsive tissues such as skeletal muscle. -
通路
Carbohydrate metabolism; hexose metabolism. -
序列相似性
Belongs to the hexokinase family.
Contains 2 hexokinase domains. -
结构域
The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule. -
细胞定位
Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding. - Information by UniProt
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别名
- DKFZp686M1669
- Hexokinase 2
- Hexokinase 2 muscle
see all -
数据库链接
- Entrez Gene: 3099 Human
- Omim: 601125 Human
- SwissProt: P52789 Human
- Unigene: 406266 Human
- Unigene: 591588 Human
相关产品
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Alternative Versions
图片
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Background-subtracted data values (mean +/- SD) are graphed.
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Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of Hexokinase II were measured in duplicates, interpolated from the Hexokinase II standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Hexokinase II concentration was determined to be 7,009.26 pg/mL in neat serum, 7,887.82 pg/mL in neat plasma (citrate), 8,052.65 pg/mL in neat plasma (EDTA), and 8,675.63 pg/mL in neat plasma (heparin).
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The concentrations of Hexokinase II were measured in duplicate and interpolated from the Hexokinase II standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Hexokinase II concentration was determined to be 1,175.25 pg/mL in placenta extract, 2,844.72 pg/mL in HeLa extract, and 2,577.06 pg/mL in Jurkat extract.
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The concentrations of Hexokinase II were measured in duplicate and interpolated from the Hexokinase II standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Hexokinase II concentration was determined to be 624.25 pg/mL in human fetal brain extract, 4,691.00 pg/mL in mouse brain extract, and 973.64 pg/mL in rat brain extract.
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To learn more about the advantages of recombinant antibodies see here.
数据表及文件
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SDS download
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Datasheet download
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