人HDAC8 ELISA试剂盒(ab282872)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.5 pg/ml
- Range: 46.88 pg/ml - 3000 pg/ml
- Sample type: Cell Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人HDAC8 ELISA试剂盒
参阅全部 HDAC8 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Extract 8 1.9% 批次间 样品 n Mean SD CV% Extract 0 0% -
样品类型
Cell Lysate -
检测类型
Sandwich (quantitative) -
灵敏度
3.5 pg/ml -
范围
46.88 pg/ml - 3000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell Lysate 101 98% - 107% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human HDAC8 ELISA kit (ab282872) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human HDAC8 protein in human cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human HDAC8 with 3.5 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
HDAC8 (Histone deacetylase 8) is a nuclear enzyme responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC8 is also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. Mouse and rat HDAC8 share 96% amino acid sequence identity with human HDAC8 protein.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Human HDAC8 Capture Antibody 1 x 600µl 10X Human HDAC8 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI2 1 x 6ml Human HDAC8 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility. -
组织特异性
Weakly expressed in most tissues. Expressed at higher level in heart, brain, kidney and pancreas and also in liver, lung, placenta, prostate and kidney. -
序列相似性
Belongs to the histone deacetylase family. HD type 1 subfamily. -
翻译后修饰
Phosphorylated by PKA on serine 39. Phosphorylation reduces deacetylase activity observed preferentially on histones H3 and H4. -
细胞定位
Nucleus. Cytoplasm. Excluded from the nucleoli. Found in the cytoplasm of cells showing smooth muscle differentiation. - Information by UniProt
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别名
- CDA07
- CDLS5
- HD 8
see all -
数据库链接
- Entrez Gene: 55869 Human
- Omim: 300269 Human
- SwissProt: Q9BY41 Human
- Unigene: 310536 Human
图片
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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The HDAC8 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of HDAC8 were measured in duplicate and interpolated from the HDAC8 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HDAC8 concentration was determined to be 2,107 pg/mL in K562 extract and 2,289 pg/mL in HeLa extract.
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The concentrations of HDAC8 were measured in duplicate and interpolated from the HDAC8 standard curve and expressing the interpolated concentrations as pg of protein per µg of extract (mean +/- SD, n=2). The mean HDAC8 concentration was determined to be 1.09 pg/µg in wild type HeLa extract and undetectable in HDAC8 knockout HeLa extract.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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