人Growth Factor抗体阵列-膜(41 Targets) (ab134002)
概述
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产品名称
人Growth Factor抗体阵列-膜(41 Targets)
参阅全部 Growth Factor Antibody Array 抗体芯片 -
样品类型
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media -
检测类型
Semi-quantitative -
种属反应性
与反应: Human -
产品概述
ab134002 is for simultaneous detection of 41 Human Growth Factors. Suitable for all sample types.
Targets: Amphiregulin, bFGF, EGF, EGF R, FGF-4, FGF-6, FGF-7, GCSF, GDNF, GM-CSF, HB-EGF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGF-I, IGF-I SR, IGF-II, M-CSF, M-CSF R, beta-NGF, NT-3, NT-4, PDGF Ra, PDGF Rß, PDGF-AA, PDGF-AB, PDGF-BB, PLGF, SCF, SCF R, TGF-alpha, TGF-beta, TGF-beta2, TGF-beta3, VEGF-A, VEGF R2, VEGF R3, VEGF-D
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
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说明
If you are interested in this cytokine array, array ab133998 may also be of interest.
A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: Multiplex Protein Detectionmore details
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 4 Membranes 1 x 8 Membranes 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 20X Wash Buffer I 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit Biotin-Conjugated Anti-Cytokines 2 vials 4 vials 1X Blocking Buffer 1 x 25ml 2 x 25ml Detection Buffer C 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml Growth Factor Antibody Array Membranes 4 units 8 units -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134002于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Multiplex Protein Detection |
Use at an assay dependent concentration.
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说明 |
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Multiplex Protein Detection
Use at an assay dependent concentration. |
图片
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MCF7 cells (Human breast adenocarcinoma) were seeded at 1x106 cells/mL and cultured in EMEM media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate. Jurkat cells (Human T lymphocytes) were seeded at 1x106 cells/mL and cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate.
Cells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 µg of total protein was assayed using ab134002.
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Cells were cultured for 48 hours, washed and harvested. Cell and tissue extracts were prepared and 250 µg of total protein was assayed using ab134002. Mean pixel density was quantified using CCD camera software analysis.
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HepG2 cells (Human hepatocellular carcinoma) were seeded at 1x106 cells/mL and cultured in EMEM media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate.
Conditioned media was harvested after 48 hours, aliquoted and assayed using ab134002.
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Conditioned media was harvested after 48 hours, aliquoted and assayed using ab134002. Mean pixel density was quantified using CCD camera software analysis.
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Human serum from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134002.
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Human serum from a pooled donor (n=50) sample was diluted to 25% and assayed using ab134002. Mean pixel density was quantified using CCD camera software analysis.
数据表及文件
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SDS download
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Datasheet download
文献 (21)
ab134002 被引用在 21 文献中.
- Yang KS et al. Propofol induces apoptosis and ameliorates 5‑fluorouracil resistance in OSCC cells by reducing the expression and secretion of amphiregulin. Mol Med Rep 25:N/A (2022). PubMed: 34859260
- Iriyama S et al. Regeneration of collagen fibrils at the papillary dermis by reconstructing basement membrane at the dermal-epidermal junction. Sci Rep 12:795 (2022). PubMed: 35039587
- Al Hrout A et al. Modelling liver cancer microenvironment using a novel 3D culture system. Sci Rep 12:8003 (2022). PubMed: 35568708
- Kim HS et al. Effect of Decellularized Extracellular Matrix Bioscaffolds Derived from Fibroblasts on Skin Wound Healing and Remodeling. Front Bioeng Biotechnol 10:865545 (2022). PubMed: 35845393
- An SY et al. Keratin-mediated hair growth and its underlying biological mechanism. Commun Biol 5:1270 (2022). PubMed: 36402892