人gamma-synuclein ELISA试剂盒(ab224884)
概述
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产品名称人gamma-synuclein ELISA试剂盒
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检测方法Colorimetric
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精确度
批次内 样品 n Mean SD CV% Serum 8 6.1% 批次间 样品 n Mean SD CV% Serum 3 14% -
样品类型Urine, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
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检测类型Semi-quantitative
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灵敏度5.15 pg/ml
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范围7.81 pg/ml - 500 pg/ml
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回收率
特定样本回收率 样品类型 平均% 范围 Urine 107 100% - 114% Serum 100 90% - 105% Heparin Plasma 89 83% - 92% EDTA Plasma 73 66% - 77% Citrate Plasma 79 75% - 84% -
检测时间1h 30m
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实验步骤One step assay
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种属反应性与反应: Human
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产品概述
Gamma-synuclein in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Gamma-synuclein protein in Human Serum, Plasmas, Urine and Cell Extracts
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Human gamma-synuclein plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway.
Sensitivity
Samples diluted in Sample Diluent NS - 5.23 ng/mL
Samples diluted in Sample Diluent PTR - 5.15 ng/mL
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经测试应用适用于: Sandwich ELISAmore details
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平台Pre-coated microplate (12 x 8 well strips)
性能
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存放说明Store at +4°C. Please refer to protocols.
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组件 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Human gamma-synuclein Capture Antibody 10X 1 x 600µl Human gamma-synuclein Detector Antibody 10X 1 x 600µl Human gamma-synuclein Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway.
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组织特异性Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung.
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序列相似性Belongs to the synuclein family.
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翻译后修饰Phosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases.
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细胞定位Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle.
- Information by UniProt
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别名
- BCSG1
- Breast cancer specific gene 1 protein
- Breast cancer specific protein 1
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数据库链接
- Entrez Gene: 6623 Human
- Omim: 602998 Human
- SwissProt: O76070 Human
- Unigene: 349470 Human
应用
Our Abpromise guarantee covers the use of ab224884 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
图片
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Example of human gamma-synuclein standard curve in Sample Diluent NSThe gamma-synuclein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human gamma-synuclein standard curve in Sample Diluent PTRThe gamma-synuclein standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native gamma-synuclein in human serum and plasma samplesThe concentrations of gamma-synuclein were measured in duplicate, interpolated from the gamma-synuclein standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean gamma-synuclein concentration was determined to be 126.89 pg/mL in serum, 114.40 pg/mL in plasma (citrate), 78.63 pg/mL in plasma (EDTA) and 173.43 pg/ml in plasma (heparin).
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Interpolated concentrations of native gamma-synuclein in human urine (from a bladder cancer patient) and cell culture extract based on a 5 µg/mL loadThe concentrations of gamma-synuclein were measured in duplicate and interpolated from the gamma-synuclein standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean gamma-synuclein concentration was determined to be 2193.46 pg/mL in bladder cancer urine, 316.85 pg/ml in HT-29 cell extract.
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Serum from ten individual healthy human female donors was measured in duplicateInterpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean gamma-synuclein concentration was determined to be 180.4 pg/mL with a range of 32.92 - 451.67 pg/mL.
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Urine from pooled normal human and four individual bladder cancer patients (male: n=3; female: n=1) human donors was measured in duplicateInterpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean gamma-synuclein concentration was determined to be 569.88 pg/mL with a range of 40.46 – 2021.56 pg/ml. The native concentration of gamma-synuclein was below the level of detection in urine from healthy human donors.
实验方案
文献
ab224884 has not yet been referenced specifically in any publications.
