人FGF19 ELISA试剂盒(ab230943)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.4 pg/ml
- Range: 3.91 pg/ml - 250 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人FGF19 ELISA试剂盒
参阅全部 FGF19 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Supernatant 5 3.8% 批次间 样品 n Mean SD CV% Supernatant 3 6.6% -
样品类型
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
1.4 pg/ml -
范围
3.91 pg/ml - 250 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 112 106% - 117% Cell culture media 108 107% - 110% Hep Plasma 108 101% - 116% EDTA Plasma 94% - 99% Cit plasma 100 97% - 105% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human
不与反应: Cow -
产品概述
Human FGF19 ELISA Kit (ab230943) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FGF19 protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human FGF19 with 1.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Human FGF19 Capture Antibody 1 x 600µl 10X Human FGF19 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human FGF19 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB. -
组织特异性
Expressed in fetal brain, cartilage, retina, and adult gall bladder. -
序列相似性
Belongs to the heparin-binding growth factors family. -
细胞定位
Secreted. - Information by UniProt
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别名
- FGF 19
- FGF-19
- FGF15
see all -
数据库链接
- Entrez Gene: 9965 Human
- NCBI: NM_005117 Human
- Omim: 603891 Human
- SwissProt: O95750 Human
- Unigene: 249200 Human
相关产品
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Alternative Versions
图片
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Standard curve comparison between human FGF19 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of FGF19 were measured in duplicates, interpolated from the FGF19 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (EDTA) 12.5%, plasma (heparin) 12.5% and plasma (citrate) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF19 concentration was determined to be 153 pg/mL in serum , 91 pg/mL in plasma (EDTA), 187 pg/mL in plasma (heparin), and 182 pg/mL in plasma (citrate).
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The concentrations of FGF19 were measured in duplicates, interpolated from the FGF19 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (citrate) 12.5%, plasma (EDTA) 12.5% and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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The concentrations of FGF19 were measured in duplicates, interpolated from the FGF19 standard curves and corrected for sample dilution. Undiluted samples are as follows: HT-29 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF19 concentration was determined to be 741 pg/mL in HT-29 cell culture supernatant.
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Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF19 concentration was determined to be 381 pg/mL with a range of 136 – 944 pg/mL.
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To learn more about the advantages of recombinant antibodies see here.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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