人FGF1 ELISA试剂盒(ab219636)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.97 pg/ml
- Range: 18.75 pg/ml - 1200 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, Saliva, Serum, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人FGF1 ELISA试剂盒
参阅全部 FGF1 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% extract 8 3.4% 批次间 样品 n Mean SD CV% extract 3 3.8% -
样品类型
Cell culture supernatant, Saliva, Urine, Serum, Tissue Extracts, Cell culture media -
检测类型
Sandwich (quantitative) -
灵敏度
1.97 pg/ml -
范围
18.75 pg/ml - 1200 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Saliva 88 87% - 91% Urine 81 78% - 83% Serum 91 89% - 94% Tissue Extracts 103 101% - 104% Cell culture media 114 112% - 115% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human FGF1 ELISA Kit (ab219636) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FGF1 protein in cell culture supernatant, saliva, serum, tissue extracts, urine, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human FGF1 with 1.97 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
Fibroblast growth factor 1 (FGF1) is a heparin-binding fibroblast growth factor that plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Specifically, FGF1 binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation. Endogenous FGF-1 is found in the nucleus of most cell types. Nuclear localization is required for FGF-1 mitogenic activity. FGF-1 promotes tumor development by promoting cancer cell proliferation and survival. Mouse and rat FGF1 are 95.5% identical to human FGF1.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
平台
Microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Human FGF1 Capture Antibody 1 x 600µl 10X Human FGF1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human FGF1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
The heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro. -
序列相似性
Belongs to the heparin-binding growth factors family. -
细胞定位
Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1. - Information by UniProt
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别名
- Acidic fibroblast growth factor
- aFGF
- Beta endothelial cell growth factor
see all -
数据库链接
- Entrez Gene: 2246 Human
- Omim: 131220 Human
- SwissProt: P05230 Human
- Unigene: 483635 Human
相关产品
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Alternative Versions
图片
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Background-subtracted data values (mean +/- SD) are graphed.
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Background-subtracted data values (mean +/- SD) are graphed.
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Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of FGF1 were measured in duplicates, interpolated from the FGF1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, urine 50%, saliva 50%, and stimulated PBMC media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF1 concentration was determined to be 868 pg/mL in neat serum, 1,275 pg/mL in neat urine, 903 pg/mL in neat saliva, and 518 pg/mL in neat stimulated PBMC culture media.
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The concentrations of FGF1 were measured in duplicate and interpolated from the FGF1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF1 concentration was determined to be 886 pg/mL in human brain extract, 179 pg/mL in mouse brain extract, and 68 pg/mL in rat brain extract.
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The concentrations of FGF1 were measured in duplicate and interpolated from the FGF1 standard curve. The interpolated values are plotted (mean +/- SD, n=2). The mean FGF1 concentration was determined to be 445 pg/mL in human brain extract, 161 pg/mL in mouse brain extract, and 61 pg/mL in rat brain extract.
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The concentrations of FGF1 were measured in duplicates and interpolated from the FGF1 standard curves. Undiluted samples are as follows: unstimulated PBMC media 100% and stimulated PBMC media 100%. The interpolated values are plotted (mean +/- SD, n=2). The level of FGF1 in neat unstimulated PBMC media was below the 7th point of the standard curve and could not be interpolated. The mean FGF1 concentration was determined to be 27 pg/mL in neat stimulated PBMC media. PBMC media samples were cultured in RPMI media with 10% fetal bovine serum (unstimulated) for 24 hours and then stimulated for 48 hours with 1.5% PHAM.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab219636 被引用在 3 文献中.
- Glorieux C et al. Regulation of PD-L1 expression in K-ras-driven cancers through ROS-mediated FGFR1 signaling. Redox Biol 38:101780 (2021). PubMed: 33171331
- Chen J et al. miR-628 reduces prostate cancer proliferation and invasion via the FGFR2 signaling pathway. Exp Ther Med 18:1005-1012 (2019). PubMed: 31316598
- Fei J et al. Low concentration of rutin treatment might alleviate the cardiotoxicity effect of pirarubicin on cardiomyocytes via activation of PI3K/AKT/mTOR signaling pathway. Biosci Rep 39:N/A (2019). PubMed: 31138757