人FAS Ligand ELISA试剂盒(CD95L) (ab45892)
Key features and details
- Sensitivity: 12 pg/ml
- Range: 62.5 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人FAS Ligand ELISA试剂盒(CD95L)
参阅全部 Fas Ligand 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Sample A 40 588.4pg/ml 22.6 3.8% Sample B 35 302.2pg/ml 14.6 4.5% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 12 pg/ml -
范围
62.5 pg/ml - 2000 pg/ml -
检测时间
3h 45m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Human FAS Ligand in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of FAS Ligand in Human serum, plasma, buffered solutions and cell culture media.
A monoclonal antibody specific for FAS Ligand has been coated onto the wells of the microtiter strips provided. Samples, including standards of known FAS Ligand concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for FAS Ligand are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of FAS Ligand present in the samples.
This kit will recognize both endogenous and recombinant Human FAS Ligand.
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平台
Microplate
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml Biotinylated anti-FAS Ligand Red 1 x 400µl 2 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml Control Silver 2 vials 4 vials FAS Ligand Microplate (12 x 8 well strips) 1 unit 2 units FAS Ligand Standard (Lyophilized) Yellow 2 vials 4 vials HRP Diluent Red 1 x 12ml 1 x 23ml Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
研究领域
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相关性
Fas / APO1 (CD95) is an important member of the tumour necrosis factor (TNF) superfamily involved in membrane-mediated apoptosis. Ligation of Fas by Fas Ligand or an anti Fas cross linking antibody, triggers activation of the caspase cascade. Functional impairment of the Fas / Fas Ligand system is associated with the development and progression of malignancies. Fas gene mutations have been suggested to have a role in testicular germ cell tumours. Tumour cells frequently exhibit de novo expression of Fas Ligand, which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells. The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/Fas Ligand system. -
细胞定位
Cell Membrane (single-pass type II membrane protein) and Secreted -
别名
- Apoptosis (APO 1) antigen ligand 1
- Apoptosis antigen ligand
- APT1LG1
see all -
数据库链接
- Entrez Gene: 356 Human
- Omim: 134638 Human
- SwissProt: P48023 Human
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab45892 被引用在 1 文献中.
- Qiu M et al. Transforming growth factor ß1 and Fas ligand synergistically enhance immune tolerance in dendritic cells in liver transplantation. J Surg Res 218:180-193 (2017). PubMed: 28985848