人Fas ELISA试剂盒(ab100513)
Key features and details
- Sensitivity: 5 pg/ml
- Range: 2.74 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Fas ELISA试剂盒
参阅全部 Fas 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 5 pg/ml -
范围
2.74 pg/ml - 2000 pg/ml -
回收率
> 85 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 91.24 82% - 105% Serum 84.46 78% - 102% Plasma 86.37 80% - 104% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Fas Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Fas in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human Fas coated on a 96-well plate. Standards and samples are pipetted into the wells and Fas present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Fas antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fas bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human FASLG Receptor ELISA Kit (CD95) (ab183360) from our SimpleStep ELISA® range.
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说明
Optimization may be required with urine samples
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 640X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human Fas 2 vials Fas Microplate (12 strips x 8 wells) 1 unit Recombinant Human Fas Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). -
组织特异性
Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. -
疾病相关
Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly. -
序列相似性
Contains 1 death domain.
Contains 3 TNFR-Cys repeats. -
结构域
Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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别名
- ALPS 1A
- ALPS1A
- APO 1
see all -
数据库链接
- Entrez Gene: 355 Human
- Omim: 134637 Human
- SwissProt: P25445 Human
- Unigene: 244139 Human
相关产品
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SimpleStep ELISA kits
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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