概述

  • 产品名称
    人FADD ELISA试剂盒
    参阅全部 FADD 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 5 2.8%
    批次间
    样品 n Mean SD CV%
    Overall 3 4.8%
  • 样品类型
    Cell culture extracts
  • 检测类型
    Sandwich (quantitative)
  • 灵敏度
    22.3 pg/ml
  • 范围
    94 pg/ml - 6000 pg/ml
  • 回收率

    特定样本回收率
    样品类型 平均% 范围
    Cell culture extracts 101 99% - 102%

  • 检测时间
    1h 30m
  • 实验步骤
    One step assay
  • 种属反应性
    与反应: Human
    不与反应: Mouse, Rat
  • 产品概述

    FADD in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of FADD protein in human cell extracts.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • 说明

    FADD (FAS-associated death domain protein) is an adaptor protein involved in recruitment of caspases to activated Fas or TNFR-1 receptors during apoptosis. It is also involved in interferon signalling during an antiviral immune response.

  • 经测试应用
    适用于: Sandwich ELISAmore details
  • 平台
    Pre-coated microplate (12 x 8 well strips)

性能

  • 存放说明
    Store at +4°C. Please refer to protocols.
  • 组件 1 x 96 tests
    10X Human FADD Capture Antibody 1 x 600µl
    10X Human FADD Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CPR 1 x 6ml
    Human FADD Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • 研究领域
  • 功能
    Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
  • 组织特异性
    Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
  • 序列相似性
    Contains 1 death domain.
    Contains 1 DED (death effector) domain.
  • 结构域
    Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
  • Information by UniProt
  • 别名
    • FADD
    • FADD protein
    • FADD_HUMAN
    • Fas (TNFRSF6) associated via death domain
    • Fas associated via death domain
    • Fas associating death domain containing protein
    • Fas associating protein
    • Fas associating protein with death domain
    • Fas TNFRSF6 associated via death domain
    • FAS-associated death domain protein
    • FAS-associating death domain-containing protein
    • GIG 3
    • GIG3
    • Growth inhibiting gene 3 protein
    • Growth-inhibiting gene 3 protein
    • H sapiens mRNA for mediator of receptor induced toxicity
    • Mediator of receptor induced toxicity
    • MGC8528
    • MORT 1
    • MORT1
    • Protein FADD
    see all
  • 数据库链接

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应用

Our Abpromise guarantee covers the use of ab214026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use at an assay dependent concentration.

图片

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native FADD in human MCF7, human Jurkat, and human liver cell extracts based on a 5 (MCF7), 10 (Jurkat) or 100 (human liver) μg/mL extract load.  The concentrations of FADD were measured in duplicate and interpolated from the FADD standard curve and corrected for sample dilution.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).  The mean FADD concentration was determined to be 4,003 pg/mL in MCF7 cell extracts, 4,275 pg/mL in Jurkat cell extracts, 1,457 pg/mL in human liver extracts.

实验方案

文献

ab214026 has not yet been referenced specifically in any publications.

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