Key features and details
- Sensitivity: 2 pg/ml
- Range: 1.37 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
产品名称人Eotaxin 2 ELISA试剂盒(CCL24)
参阅全部 Eotaxin 2 试剂盒
样品类型Cell culture supernatant, Serum, Plasma
灵敏度< 2 pg/ml
范围1.37 pg/ml - 1000 pg/ml
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 95.77 82% - 103% Serum 94.86 83% - 103% Plasma 96.74 85% - 104%
实验步骤Multiple steps standard assay
Abcam’s Eotaxin 2 (CCL24) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Eotaxin 2 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human Eotaxin 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Eotaxin 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Eotaxin 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Eotaxin 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human Eotaxin-2 ELISA Kit (CCL24) (ab179882) from our SimpleStep ELISA® range.
Optimization may be required with urine samples.
存放说明Store at -20°C. Please refer to protocols.
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human Eotaxin 2 2 vials Eotaxin 2 Microplate (12 x 8 well strips) 1 unit Recombinant Human Eotaxin 2 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
相关性Eotaxin 2 is a member of the CC chemokine family, based on the presence of the CC motif and homology with other known CC chemokines. Eotaxin 2 cDNA encodes a 119 amino acid residue precursor protein with a 26 amino acid residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N glycosylation site. Mature human eotaxin 2 has a predicted molecular mass of approximately 10.6 kDa. Compared to other CC chemokines, eotaxin 2 exhibits 40 %, 42 %, and 39 % amino acid identity to MCP3, MIP1 alpha, and eotaxin, respectively. Human CC chemokine eotaxin 2 maps to chromosome 7q11.23. Both eotaxin and eotaxin 2 activate and attract eosinophils and basophils. A receptor for human eotaxin has been identified and found to be the third numbered receptor in the C-C chemokine subfamily of receptors (CCR3. On eosinophils, the effects of eotaxin 2 is inhibited by an CCR3 antibody and cross-desensitized by eotaxin and MCP4, suggesting that all three CC chemokines act through CCR3. Eotaxin 2 mRNA is weakly expressed in activated monocytes and T lymphocytes. Recombinant eotaxin 2 induces chemotaxis of eosinophils, basophils, and resting T lymphocytes but not monocytes and activated T lymphocytes. Eotaxin 2 inhibits colony formation in myleloid progenitor cells.
- C C motif chemokine 24
- Chemokine (C C motif) ligand 24
ab100509 被引用在 1 文献中.
- Segal-Salto M et al. A blocking monoclonal antibody to CCL24 alleviates liver fibrosis and inflammation in experimental models of liver damage. JHEP Rep 2:100064 (2020). PubMed: 32039405