人CXCL9 ELISA试剂盒(ab100595)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 8.23 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人CXCL9 ELISA试剂盒
参阅全部 CXCL9 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 20 pg/ml -
范围
8.23 pg/ml - 6000 pg/ml -
回收率
97 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 101.3 89% - 108% Serum 94.56 82% - 102% Plasma 96.32 84% - 103% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s CXCL9 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CXCL9 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human CXCL9 coated on a 96-well plate. Standards and samples are pipetted into the wells and CXCL9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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说明
Optimization may be required with urine samples.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human CXCL9 2 vials CXCL9 Microplate (12 x 8 wells) 1 unit Recombinant Human CXCL9 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3. -
序列相似性
Belongs to the intercrine alpha (chemokine CxC) family. -
细胞定位
Secreted. - Information by UniProt
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别名
- C-X-C motif chemokine 9
- chemokine (C-X-C motif) ligand 9
- CMK
see all -
数据库链接
- Entrez Gene: 4283 Human
- Omim: 601704 Human
- SwissProt: Q07325 Human
- Unigene: 77367 Human
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab100595 被引用在 2 文献中.
- Yu J et al. Targeting macrophage priming by polyphyllin VII triggers anti-tumor immunity via STING-governed cytotoxic T-cell infiltration in lung cancer. Sci Rep 10:21360 (2020). PubMed: 33288772
- Ren X et al. Effects of fluconazole on the clinical outcome and immune response in fungal co-infected tuberculosis patients. Microb Pathog 117:148-152 (2018). ELISA . PubMed: 29432913