人Chemokine抗体阵列-膜(38 targets) (ab169812)
概述
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产品名称
人Chemokine抗体阵列-膜(38 targets)
参阅全部 Chemokine Antibody Array 抗体芯片 -
样品类型
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media -
检测类型
Semi-quantitative -
种属反应性
与反应: Human -
产品概述
ab169812 is for the simultaneous detection 38 Human Chemokine Factors. Suitable for all liquid sample types.
Targets: BLC / CXCL13, CCL28, Ck beta 8-1 / CCL23, CTACK, CXCL16, ENA-78 / CXCL5, Eotaxin-1 / CCL11, Eotaxin-2 / CCL24, Eotaxin-3 / CCL26, Fractalkine / CX3CL1, GCP-2 / CXCL6, GRO / CXCL1+2+3, GRO alpha / CXCL1, HCC-4 / CCL16, I-309 / CCL1, I-TAC / CXCL11, IL-8 / CXCL8, IP-10 / CXCL10, Lymphotactin / XCL1, MCP-1 / CCL2, MCP-2 / CCL8, MCP-3 / CCL7, MCP-4 / CCL13, MDC / CCL22, MIG / CXCL9, MIP-1 alpha / CCL3, MIP-1 beta / CCL4, MIP-1 delta / CCL15, MIP-3 alpha / CCL20, MIP-3 beta / CCL19, MPIF-1 / CCL23, NAP-2 / CXCL7 / PPBP, PARC / CCL18, RANTES / CCL5, SDF-1 alpha / CXCL12alpha, SDF-1 beta / CXCL12beta, TARC / CCL17, TECK / CCL25
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
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说明
If you are interested in this cytokine array, array ab133998 may also be of interest.
A table listing all of our human membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: Multiplex Protein Detectionmore details
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 4 Membranes 1 x 8 Membranes 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 1X Blocking Buffer 1 x 25ml 2 x 25ml 2000X Biotin-Conjugated Anti Cytokines 2 vials 4 vials 20X Wash Buffer I 1 x 10ml 1 x 20ml 20X Wash Buffer II 1 x 10ml 1 x 20ml 2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit Chemokine Antibody Array Membranes 4 units 8 units Detection Buffer C 1 x 1.5ml 1 x 2.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab169812于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Multiplex Protein Detection |
Use at an assay dependent concentration.
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说明 |
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Multiplex Protein Detection
Use at an assay dependent concentration. |
图片
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab169812 被引用在 2 文献中.
- Workel HH et al. A Transcriptionally Distinct CXCL13+CD103+CD8+ T-cell Population Is Associated with B-cell Recruitment and Neoantigen Load in Human Cancer. Cancer Immunol Res 7:784-796 (2019). PubMed: 30872264
- Sato Y et al. Elimination of LMP1-expressing cells from a monolayer of gastric cancer AGS cells. Oncotarget 8:39345-39355 (2017). PubMed: 28454117