Key features and details
- Sensitivity: 20 pg/ml
- Range: 20.58 pg/ml - 15000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
样品类型Cell culture supernatant, Serum, Plasma
灵敏度< 20 pg/ml
范围20.58 pg/ml - 15000 pg/ml
> 83 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 83.49 75% - 90% Serum 96.92 82% - 112% Plasma 86.5 70% - 105%
实验步骤Multiple steps standard assay
Abcam’s CEACAM1 (BGP) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CEACAM1 in serum, plasma, cell culture supernatant.
This assay employs an antibody specific for Human CEACAM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and CEACAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CEACAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CEACAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
存放说明Store at -20°C. Please refer to protocols.
组件 1 x 96 tests 400X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human CEACAM1 2 vials CEACAM1 Microplate (12 x 8 well strips) 1 unit Recombinant Human CEACAM1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
相关性CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glyco-protein (PSG 1-PSG 11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/ CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria.
- Antigen CD66
- BGP 1
- SwissProt: P13688 Human