人CD3 Epsilon ELISA试剂盒(ab277708)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 38.1 pg/ml
- Range: 84 pg/ml - 5400 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人CD3 Epsilon ELISA试剂盒
参阅全部 CD3 epsilon 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Extract 8 2.7% 批次间 样品 n Mean SD CV% Extract 4 6.3% -
样品类型
Cell culture supernatant, Serum, Cell Lysate -
检测类型
Sandwich (quantitative) -
灵敏度
38.1 pg/ml -
范围
84 pg/ml - 5400 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 89 83% - 101% Serum 115 114% - 116% Cell Lysate 110 105% - 120% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human CD3 Epsilon ELISA kit (ab277708) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human CD3 Epsilon in human serum, cell culture supernatant, and cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD3 Epsilon with 38.1 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
T-Cell Surface Glycoprotein CD3 epsilon chain (CD3e), also known as T-cell surface antigen T3/Leu-4 epsilon is a type I membrane protein encoded by the CD3E gene. CD3e forms the T-cell receptor (TCR)-CD3 complex on T-lymphocyte cell surfaces by forming two heterodimers CD3E/CD3D (delta) and CD3E/CD3G (gamma). This complex plays a role in coupling antigen recognition to several intracellular signal-transduction pathways. CD3e contains an immunoreceptor tyrosine-based activation motif (ITAM) in the cytoplasmic domain. When engaged by TCR, these motifs are phosphorylated by Src protein family kinases LCK and FYN, which activate downstream signaling pathways.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Human CD3 epsilon Capture Antibody 1 x 600µl 10X Human CD3 epsilon Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI2 1 x 6ml Human CD3 epsilon Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
The CD3 complex mediates signal transduction. -
序列相似性
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
细胞定位
Membrane. - Information by UniProt
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别名
- CD3 epsilon
- CD3e
- CD3e antigen
see all -
数据库链接
- Entrez Gene: 916 Human
- Omim: 186830 Human
- SwissProt: P07766 Human
- Unigene: 3003 Human
相关产品
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Alternative Versions
图片
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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The CD3 epsilon standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The CD3 epsilon standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of CD3 epsilon were measured in duplicate and interpolated from the CD3 epsilon standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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To learn more about the advantages of recombinant antibodies see here.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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