人CCL23 ELISA试剂盒(ab100611)
Key features and details
- Sensitivity: 7 pg/ml
- Range: 8.23 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人CCL23 ELISA试剂盒
参阅全部 CCL23 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 7 pg/ml -
范围
8.23 pg/ml - 6000 pg/ml -
回收率
93 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 93.48 81% - 105% Serum 92.78 82% - 105% Plasma 94.16 83% - 106% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s CCL23 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CCL23 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human CCL23 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL23 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CCL23 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL23 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human CCL23 ELISA Kit (ab216169) from our SimpleStep ELISA® range.
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说明
Optimisation may be required with urine samples.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human CCL23 2 vials CCL23 Microplate (12 x 8 wells) 1 unit Recombinant Human CCL23 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Shows chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23. -
组织特异性
High levels in adult lung, liver, skeletal muscle and pancreas. Moderate levels in fetal liver, adult bone marrow and placenta. The short form is the major species and the longer form was detected only in very low abundance. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are found in high levels in synovial fluids from rheumatoid patients. -
序列相似性
Belongs to the intercrine beta (chemokine CC) family. -
细胞定位
Secreted. - Information by UniProt
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别名
- C C motif chemokine ligand 23
- C-C motif chemokine 23
- C6 beta chemokine
see all -
数据库链接
- Entrez Gene: 6368 Human
- Omim: 602494 Human
- SwissProt: P55773 Human
- Unigene: 169191 Human
相关产品
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SimpleStep ELISA kits
图片
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Typical Standard Curve
Representative Standard Curve using ab100611.
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Typical Standard CurveRepresentative Standard Curve using ab100611.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab100611 被引用在 1 文献中.
- Yu K et al. A multi-stage association study of plasma cytokines identifies osteopontin as a biomarker for acute coronary syndrome risk and severity. Sci Rep 9:5121 (2019). PubMed: 30914768