人Cathepsin D ELISA试剂盒(ab119586)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Cathepsin D ELISA试剂盒
参阅全部 Cathepsin D 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts -
检测类型
Sandwich (quantitative) -
灵敏度
< 10 pg/ml -
范围
156 pg/ml - 10000 pg/ml -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Human Cathepsin D in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Cathepsin D in cell culture supernatants, serum and plasma (heparin, EDTA).
A Cathepsin D specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for Cathepsin D is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human Cathepsin D amount of sample captured in plate.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 标识符 1 x 96 tests 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml 1 x 12ml Anti-Human Cathepsin D antibody Microplate (12 x 8 wells) 1 unit 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl 1 x 100µl Biotinylated anti-Human Cathepsin D antibody 1 x 100µl 1 x 100µl Lyophilized recombinant Human Cathepsin D standard 2 x 10ng 2 x 10ng Plate Seal 1 x 4 units 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml 1 x 10ml Wash Buffer (25X) 1 x 20ml 1 x 20ml -
研究领域
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功能
Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. -
组织特异性
Expressed in the aorta extrcellular space (at protein level). -
疾病相关
Ceroid lipofuscinosis, neuronal, 10 -
序列相似性
Belongs to the peptidase A1 family.
Contains 1 peptidase A1 domain. -
翻译后修饰
N- and O-glycosylated. -
细胞定位
Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380). - Information by UniProt
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别名
- CatD
- CATD_HUMAN
- Cathepsin D
see all -
数据库链接
- Entrez Gene: 1509 Human
- Omim: 116840 Human
- SwissProt: P07339 Human
- Unigene: 654447 Human
图片
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Cathepsin D measured in biological fluids and cell culture medium with background signal subtracted (duplicates +/- SD). We recommend to test the human plasma, serum and urine samples in the range of 1:20-1:500, the saliva in the range of 1:100-1:1000 and the cell culture supernatants 1:1-1:1000.
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Representative Standard Curve using ab119586.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab119586 被引用在 6 文献中.
- Kim J et al. Relationship between lip skin biophysical and biochemical characteristics with corneocyte unevenness ratio as a new parameter to assess the severity of lip scaling. Int J Cosmet Sci 43:275-282 (2021). PubMed: 33544395
- Hoes MF et al. The role of cathepsin D in the pathophysiology of heart failure and its potentially beneficial properties: a translational approach. Eur J Heart Fail 22:2102-2111 (2020). PubMed: 31797504
- Afinogenova Y et al. Aberrant progranulin, YKL-40, cathepsin D and cathepsin S in Gaucher disease. Mol Genet Metab N/A:N/A (2019). PubMed: 31358474
- Vermassen T et al. Neither creatinine- nor cystatin C-estimated glomerular filtration rate is optimal in oncology patients treated with targeted agents. Nephrol Dial Transplant 33:402-408 (2018). PubMed: 28482048
- Braconi D et al. Inflammatory and oxidative stress biomarkers in alkaptonuria: data from the DevelopAKUre project. Osteoarthritis Cartilage N/A:N/A (2018). ELISA . PubMed: 29852277
- Vital P et al. The senescence-associated secretory phenotype promotes benign prostatic hyperplasia. Am J Pathol 184:721-31 (2014). PubMed: 24434012