人BNP ELISA试剂盒(ab193694)
Key features and details
- Sensitivity: 14 pg/ml
- Range: 14 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人BNP ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Overall < 10% 批次间 样品 n Mean SD CV% Overall < 12% -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
14 pg/ml -
范围
14 pg/ml - 1000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Serum 78.12 68% - 83% Plasma 80.96 67% - 107% Cell culture media 94.31 83% - 105% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s BNP Human ELISA Kit (ab193694) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human BNP in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human BNP coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures BNP present in the samples. The wells are washed and biotinylated anti-Human BNP antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of BNP bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated Human BNP Detection antibody 2 vials Human BNP Standards (lyophilized) 2 vials Pre-coated Human BNP Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
功能
Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. -
组织特异性
Brain and also in atria, but at much lower levels than ANP. -
序列相似性
Belongs to the natriuretic peptide family. -
细胞定位
Secreted. - Information by UniProt
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别名
- ANFB_HUMAN
- BNP(1-32)
- BNP(5-29)
see all -
数据库链接
- Entrez Gene: 4879 Human
- Omim: 600295 Human
- SwissProt: P16860 Human
- Unigene: 219140 Human
图片
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab193694 被引用在 10 文献中.
- He S et al. Dapagliflozin Protects Methamphetamine-Induced Cardiomyopathy by Alleviating Mitochondrial Damage and Reducing Cardiac Function Decline in a Mouse Model. Front Pharmacol 13:925276 (2022). PubMed: 35873593
- Hamam A et al. Worsening effect of testosterone deficiency on males with heart failure with preserved ejection fraction. BMC Endocr Disord 22:321 (2022). PubMed: 36529735
- Mills RJ et al. BET inhibition blocks inflammation-induced cardiac dysfunction and SARS-CoV-2 infection. Cell 184:2167-2182.e22 (2021). PubMed: 33811809
- Stojanovic D et al. The Discriminatory Ability of Renalase and Biomarkers of Cardiac Remodeling for the Prediction of Ischemia in Chronic Heart Failure Patients With the Regard to the Ejection Fraction. Front Cardiovasc Med 8:691513 (2021). PubMed: 34395559
- Mastikhina O et al. Human cardiac fibrosis-on-a-chip model recapitulates disease hallmarks and can serve as a platform for drug testing. Biomaterials 233:119741 (2020). PubMed: 31927251
- Stojanovic D et al. The partnership between renalase and ejection fraction as a risk factor for increased cardiac remodeling biomarkers in chronic heart failure patients. Curr Med Res Opin 36:909-919 (2020). PubMed: 32297799
- Mitic VT et al. Cardiac Remodeling Biomarkers as Potential Circulating Markers of Left Ventricular Hypertrophy in Heart Failure with Preserved Ejection Fraction. Tohoku J Exp Med 250:233-242 (2020). PubMed: 32295985
- Hippensteel JA et al. Intravenous fluid resuscitation is associated with septic endothelial glycocalyx degradation. Crit Care 23:259 (2019). PubMed: 31337421
- Hu H et al. Association of Gene Polymorphisms in the Human MicroRNA-126 Gene with Plasma-Circulating MicroRNA-126 Levels and Acute Myocardial Infarction. Genet Test Mol Biomarkers 23:460-467 (2019). PubMed: 31184921
- Stojanovic D et al. Association of Plasma Renalase and Left Ventricle Mass Index in Heart Failure Patients Stratified to the Category of the Ejection Fraction: A Pilot Study. Dis Markers 2019:7265160 (2019). PubMed: 31737132