Cell culture media from BMP7-transfected HEK293T cells was used for the detection of BMP7 using the "Human BMP7 ELISA kit"
Assay procedure was followed as per manufacturer's instructions: 100uL standards and samples were added into wells in duplicate, dilutions were performed accordingly, and 96-well plate was incubated overnight. Absorbance values were read at 450nm immediately following addition of Stop Solution.
A successful standard curve was obtained, however there was modest to no detection of BMP7 in samples.
Assay procedure was followed as per manufacturer's instructions: 100uL standards and samples were added into wells in duplicate, dilutions were performed accordingly, and 96-well plate was incubated overnight. Absorbance values were read at 450nm immediately following addition of Stop Solution.
A successful standard curve was obtained, however there was modest to no detection of BMP7 in samples.
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提交于 Oct 22 2018