人beta 2 Microglobulin ELISA试剂盒(B2M) (ab99977)
Key features and details
- Sensitivity: 6 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人beta 2 Microglobulin ELISA试剂盒(B2M)
参阅全部 beta 2 Microglobulin 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 6 pg/ml -
范围
15.6 pg/ml - 1000 pg/ml -
回收率
< 100 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 104.6 93% - 118% Serum 94.28 80% - 103% Plasma 95.78 81% - 105% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s beta 2 Microglobulin (B2M) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human beta 2 Microglobulin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human beta 2 Microglobulin coated on a 96-well plate. Standards and samples are pipetted into the wells and beta 2 Microglobulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human beta 2 Microglobulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of beta 2 Microglobulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human Beta-2-Microglobulin ELISA Kit (ab181423) from our SimpleStep ELISA® range.
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说明
Optimisation may be required with urine samples.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 2 x 30ml beta 2 Microglobulin Microplate (12 x 8 wells) 1 unit Biotinylated anti-Human beta 2 Microglobulin 2 vials Recombinant Human beta 2 Microglobulin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
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功能
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. -
疾病相关
Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis. -
序列相似性
Belongs to the beta-2-microglobulin family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
翻译后修饰
Glycation of Ile-21 is observed in long-term hemodialysis patients. -
细胞定位
Secreted. Detected in serum and urine. - Information by UniProt
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别名
- B2M
- B2MG_HUMAN
- Beta 2 microglobin
see all -
数据库链接
- Entrez Gene: 567 Human
- Omim: 109700 Human
- SwissProt: P61769 Human
- Unigene: 534255 Human
- Unigene: 702947 Human
相关产品
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SimpleStep ELISA kits
图片
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab99977 被引用在 1 文献中.
- Lee SH et al. Proteome alterations in the aqueous humor reflect structural and functional phenotypes in patients with advanced normal-tension glaucoma. Sci Rep 12:1221 (2022). PubMed: 35075201