人beta 2 Microglobulin ELISA试剂盒(B2M) (ab99977)

This kit has not been tested for cross-reactivity with mouse samples. However, since human and mouse Beta-2M differ by 30% in their primary sequence it is unlikely this kit will detect mouse Beta 2M.

Thank you for contacting us.

As you mention, the kit can be stored up to 6 months at temperatures between 4-8°C.

The standard is stable at these temperatures as long as it has not been reconstituted.

Once reconstituted with the correspondent Assay Diluent it should be stored at –80°C and be used as soon as possible.

I hope this information is helpful. Please do not hesitate to contact us for further assistance.

Thank you for your enquiry and your interest in our products.

Currently, we do have 2 kits for detecting human beta 2 Microglobulin i.e. ab99977 and ab108885.

I would advise you to read through the datasheets as well as the Protocol Booklet (which can be found and down-loaded under the Protocol section) to see which one would suit your need:

https://www.abcam.com/beta-2-microglobulin-human-elisa-kit-1-x-96-well-plate-ab99977.html

https://www.abcam.com/beta-2-microglobulin-human-elisa-kit-ab108885.html

In Italy, we have an authorized distributor Prodotti Gianni, so if you have any further enquiry regarding shipment or order I would advise you to contact them directly. Here are their contact details:

http://www.prodottigianni.com

mailto:fberetta@prodottigianni.com

mailto:rsitta@prodottigianni.com

If you need any further assistance in the future, please do not hesitate to contact me.

Thank you for contacting Abcam. I have been in contact with the labs about our kit and also have some additional details regarding our other Beta-2-Microglobulin products reactivity with porcine.

For our kits:

Regardingab99977:Unfortunately, we have not tested the capture antibody against pig Beta-2-Microglobulin; however, the detection antibody is cross reactive with porcine Beta-2-Microglobulin (B2M). This does imply high homology between porcine B2M and human B2M. Therefore, there is a good possibility that the kit will successfully react with the porcine tissue sample.

It is also important to note that this kit was neither designed nor validated with lysate samples, our kits are very accommodating to many different samples types so lysate samples willlikelywork. Should you decide to test this kit with lysate samples, we recommend diluting the samples at least 5-fold with Assay DiluentBto minimize any effects of the detergents in the lysis buffer. The samples may need to be diluted further but this would need to be determined empirically.

For the original lysate, in general would want to shoot for at least 1 mg/ml total protein (preferably more) to achieve 50-500 ug/ml after dilution. Again though, this range should just be used as a starting point and the optimal concentration would need to be determined empirically.

When preparing a lysis buffer, attached are some general guidelines for doing so along with some basic tips for sample preparation. In brief, a lysis buffer must meet the following specifications:

A) has relatively low salt content (700 mM or less)
B) does not contain sodium azide
C) does not contain >0.1% SDS
D) does not contain >10 mM reducing agents (beta-mercaptoethanol or dithiothreitol)

This would include any buffers used for immunoprecipitations, including RIPA buffer.

Forab108885:We have tested porcine plasma/ serum samples with this kit. Low cross-reactivity observed so we do not suggest this kit for porcine tissue.

For our antibodies:

The Human to Porcine Identity of B2M is 72.269% overall. However there is good homology at the C terminus and in some internal regions.

Our Anti-beta 2 Microglobulin antibody [HYB332-01] (ab27155) is raised against a region with very good homology (89%). It may be found at:https://www.abcam.com/beta-2-Microglobulin-antibody-HYB332-01-ab27155.html

Anti-beta 2 Microglobulin antibody [EP2978Y] (ab75853) may be acceptable as well though as it is against the C terminus. The product page is at:https://www.abcam.com/beta-2-Microglobulin-antibody-EP2978Y-ab75853.html.

For UNTESTED species and/or applications, we have established a testing discount program. Either of these antibodies or ourbeta 2 Microglobulin Human ELISA Kit ab99977 would qualify.Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

In order to better understand the problem, I’d like to send you a questionnaire with some questions about the protocol used as well as the batch and order numbers.

Where both kits from the same batch? Were the same samples used in the two assay attempts? Did you change anything during the assay performance, as pipettes, or any other equipment?

Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. If no suggestions can be made to the protocol, and the antibody was purchased within the last 6 months, it is covered by our Abpromise guarantee, and therefore I can offer you a free of charge replacement or a credit note for it.

I look forward to receiving your reply. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your inquiry.

I can confirm that serum/plasma samples usually require a 5,000˜100,000 fold dilution. I am sorry, I am unable to give a morenarrow range.

I agree that aserial dilutionis the best approach.

For example, add 2 μl of serum/plasma into a tube with 398.0 μl Assay Diluent A to prepare a 200-fold diluted sample. Mix through and then pipette 2 μl of prepared 200-fold diluted sample into a tube with 498 μl 1x Assay Diluent A to prepare a final 50,000 fold diluted sample.

I hope this information is helpful and wish you good luck with your experiments.

Thank you for your inquiry and you positive feedback.

Please see my answers to your questions below:

1. I can confirm that the kits ab108902 (Transferrin Human ELISA Kit) and ab108788 (Albumin Human ELISA kit) were especially optimised for cell culture samples and I recommend to choose them for the experiment.

2. We do offer bulk oder discounts. If you order 5 or more products you will get a discount. For 6 products you will currently get a 5% discount. Please do not forget to mention that when ordering. If you are planning to order more than 10 products, please contact our customer service department with the numbers and they might be able to offer you a higher discount.

3. I can confirm that c stands for competitive ELISA and s stands for sandwich ELISA. In a competitive ELISA the competitor is labeled with biotin and the amount of the bound competitor is measured. This means that a high OD indicates a low amount of the protein of interest in your sample. A low OD means that the competitor has been ousted and there is a high amount of the protein of interest in the sample.

4. I can confirm that the main difference between these two kits is the sensitivity. Both are suitable for cell culture supernatant, urine, serum, plasma.

Our Abpromise guarantee applies to all these kits.

https://www.abcam.com/abpromise

I hope this information is helpful and wish you good luck with your experiments. Please do not hesitate to contact me again with any further questions.