人Apolipoprotein AI ELISA试剂盒(APOA1) (ab108804)

概述

  • 产品名称
    人Apolipoprotein AI ELISA试剂盒(APOA1)
    参阅全部 Apolipoprotein A I 试剂盒
  • 检测方法
    Colorimetric
  • 精确度
    批次内
    样品 n Mean SD CV%
    Overall 4.9%
    批次间
    样品 n Mean SD CV%
    Overall 7.2%
  • 样品类型
    Serum, Plasma
  • 检测类型
    Competitive
  • 灵敏度
    1 µg/ml
  • 范围
    0.625 µg/ml - 20 µg/ml
  • 回收率

    98 %

  • 检测时间
    3h 00m
  • 实验步骤
    Multiple steps standard assay
  • 种属反应性
    与反应: Human
  • 产品概述

    Apolipoprotein AI Human (APOA1) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Apolipoprotein AI levels in plasma and serum.


    An Apolipoprotein AI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Apolipoprotein AI is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Apolipoprotein AI captured in plate.


    Get results in 90 minutes with Human Apolipoprotein AI ELISA Kit (ab189576) from our SimpleStep ELISA® range.

  • 经测试应用
    适用于: Competitive ELISAmore details
  • 平台
    Microplate

性能

  • 存放说明
    Store at -20°C. Please refer to protocols.
  • 组件 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 1 x 30ml
    2X Biotinylated Human Apolipoprotein AI (Lyophilized) 1 vial
    Apolipoprotein AI Microplate (12 x 8 well strips) 1 unit
    Apolipoprotein AI Standard 1 vial
    Chromogen Substrate 1 x 8ml
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • 研究领域
  • 功能
    Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
  • 组织特异性
    Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease.
  • 疾病相关
    High density lipoprotein deficiency 2
    High density lipoprotein deficiency 1
    APOA1 mutations may be involved in the pathogenesis of amyloid polyneuropathy-nephropathy Iowa type, also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III (PubMed:3142462 and PubMed:2123470). The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis.
    Amyloidosis 8
  • 序列相似性
    Belongs to the apolipoprotein A1/A4/E family.
  • 翻译后修饰
    Palmitoylated.
    Met-110 and Met-136 are oxidized to methionine sulfoxides.
    Phosphorylation sites are present in the extracellular medium.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 别名
    • Apo-AI
    • ApoA-I
    • APOA1
    • APOA1_HUMAN
    • Apolipoprotein A-I
    • Apolipoprotein A-I(1-242)
    • Apolipoprotein A1
    • Truncated apolipoprotein A-I
    see all
  • 数据库链接

相关产品

应用

Our Abpromise guarantee covers the use of ab108804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Competitive ELISA Use at an assay dependent concentration.

图片

  • Apolipoprotein A1 measured in biological fluids and cell culture medium with background signal subtracted (duplicates +/- SD).
  • Representative Standard Curve using ab108804

实验方案

文献

This product has been referenced in:
  • Lin Q  et al. Decreased expression of the APOA1-APOC3-APOA4 gene cluster is associated with risk of Alzheimer's disease. Drug Des Devel Ther 9:5421-31 (2015). ELISA . Read more (PubMed: 26491253) »

See 1 Publication for this product

客户评价及客户问答

Filter by Ratings

Abreview for Apolipoprotein A1 Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Human Serum (Human plasma)
Specification: Human plasma
Amount of Sample: 2 µg
Type of Kit Used: ELISA
Positive control: recombinant apolipoprotein A1
Did you use the Abcam Kit protocol?: Yes
Username

Abcam user community

Verified customer

提交于 Jan 15 2013

Abreviews

Sample: Human
Amount of Sample: 0-20 µg
Type of Kit Used: ELISA
Positive control: APOA1, APOB, HDL, LDL
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Kit detects APOB
Abcam response: We thank the customer for this feedback and we are currently working to resolve this issue.
Username

Abcam user community

Verified customer

提交于 May 04 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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