人Annexin V ELISA试剂盒(ab223863)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 23.4 pg/ml
- Range: 46.9 pg/ml - 3000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Annexin V ELISA试剂盒
参阅全部 Annexin V/ANXA5 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Supernatant 5 3.8% 批次间 样品 n Mean SD CV% Supernatant 3 10.8% -
样品类型
Cell culture supernatant, Urine, Cell culture extracts, Tissue Extracts -
检测类型
Sandwich (quantitative) -
灵敏度
23.4 pg/ml -
范围
46.9 pg/ml - 3000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Urine 98 96% - 101% Cell culture extracts 90 86% - 96% Tissue Extracts 103 100% - 105% Cell culture media 107 99% - 114% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human -
产品概述
Human Annexin V ELISA Kit (ab223863) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Annexin V protein in cell culture extracts, cell culture supernatant, tissue extracts, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Annexin V with 23.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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说明
Annexin V is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Annexin V is a 36 kDa protein that binds to phospholipids in a calcium-dependent manner. Annexin V preferentially binds phosphatidylserine, in competition with prothrombin, leading to inhibition of blood coagulation at sites of injury preceding contact between lipid components and coagulation factors that initiate thrombosis. The ability of Annexin V to bind to phosphatidylserine makes it an attractive reagent in detecting apoptotic cells.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml 10X Human Annexin V Capture Antibody 1 x 600µl 10X Human Annexin V Detector Antibody 1 x 600µl Human Annexin V Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究领域
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功能
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. -
疾病相关
Pregnancy loss, recurrent, 3 -
序列相似性
Belongs to the annexin family.
Contains 4 annexin repeats. -
结构域
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
翻译后修饰
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
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别名
- Anchorin CII
- Annexin 5
- Annexin A5
see all -
数据库链接
- Entrez Gene: 308 Human
- Omim: 131230 Human
- SwissProt: P08758 Human
- Unigene: 480653 Human
相关产品
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Alternative Versions
图片
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Annexin V in human HepG2 cell extract based on a 1 μg/mL extract load and heart and brain tissue extract samples based on a 2 μg/mL extract load. The concentrations of Annexin V were measured in duplicate and interpolated from the Annexin V standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Annexin V concentration was determined to be 1299 pg/mL in HepG2 cell extract, 2159 pg/mL in human heart tissue extract, and 2585 pg/mL in human brain tissue extract.
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Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of Annexin V were measured in duplicates, interpolated from the Annexin V standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 20% and HepG2 cell culture supernatant 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Annexin V concentration was determined to be 1601 pg/mL in neat urine and 14,220 pg/mL in neat HepG2 Cell Culture Supernatant.
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To learn more about the advantages of recombinant antibodies see here.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab223863 被引用在 1 文献中.
- Park SE et al. Geometric engineering of organoid culture for enhanced organogenesis in a dish. Nat Methods 19:1449-1460 (2022). PubMed: 36280722