人Angiopoietin 2 ELISA试剂盒(ANG2) (ab99971)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 4.12 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
-
产品名称
人Angiopoietin 2 ELISA试剂盒(ANG2)
参阅全部 Angiopoietin 2/ANG2 试剂盒 -
检测方法
Colorimetric -
样品类型
Cell culture supernatant, Serum, Plasma -
检测类型
Sandwich (quantitative) -
灵敏度
< 10 pg/ml -
范围
4.12 pg/ml - 3000 pg/ml -
回收率
88 %
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 82.1 76% - 92% Serum 85.3 82% - 109% Plasma 96.9 95% - 116% -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
Abcam’s Angiopoietin 2 (ANG2) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Angiopoietin 2 in serum, plasma and cell culture supernates.
This assay employs an antibody specific for Human Angiopoietin 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Angiopoietin 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Angiopoietin 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Angiopoietin 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
说明
Optimisation may be required with urine samples.
-
平台
Microplate
性能
-
存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Angiopoietin 2 Microplate (12 x 8 well strip) 1 unit Biotinylated anti-Human Angiopoietin 2 2 vials Recombinant Angiopoietin 2 Standard Human (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究领域
-
功能
Can induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. -
序列相似性
Contains 1 fibrinogen C-terminal domain. -
结构域
The Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor. -
细胞定位
Secreted. - Information by UniProt
-
别名
- AGPT 2
- Agpt2
- ANG 2
see all -
数据库链接
- Entrez Gene: 285 Human
- Omim: 601922 Human
- SwissProt: O15123 Human
- Unigene: 583870 Human
数据表及文件
-
SDS download
-
Datasheet download
文献 (3)
ab99971 被引用在 3 文献中.
- Xia S et al. Abnormal expression of microRNA-575 leads to missed abortion through regulating apoptosis and angiogenesis. Exp Ther Med 14:3993-4000 (2017). PubMed: 29104621
- Zhou L et al. Plasma angiopoietin-2 is persistently elevated after non-small cell lung cancer surgery and stimulates angiogenesis in vitro. Medicine (Baltimore) 95:e4493 (2016). Human . PubMed: 27512865
- Genre F et al. Antitumour necrosis factor-a therapy modulates angiopoietin-2 serum levels in non-diabetic ankylosing spondylitis patients. Ann Rheum Dis 72:1265-7 (2013). PubMed: 23482472