Key features and details
- Sensitivity: 10 pg/ml
- Range: 16.46 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
参阅全部 Amphiregulin 试剂盒
样品类型Cell culture supernatant, Plasma
灵敏度< 10 pg/ml
范围16.46 pg/ml - 4000 pg/ml
特定样本回收率 样品类型 平均% 范围 Cell culture supernatant 100.4 89% - 111% Plasma 72.7 64% - 87%
实验步骤Multiple steps standard assay
Abcam’s Amphiregulin (AREG) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Amphiregulin in plasma, and cell culture supernatants. (Human Amphiregulin concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human Amphiregulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Amphiregulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Amphiregulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Amphiregulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Human Amphiregulin in normal serum with ab99975, only in serum spiked with Human Amphiregulin.
Optimisation may be required with urine samples.
存放说明Store at -20°C. Please refer to protocols.
组件 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Amphiregulin Microplate (12 x 8 well strips) 1 unit Assay Diluent A 1 x 30ml Biotinylated anti-Human Amphiregulin 2 vials Recombinant Human Amphiregulin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
相关性The mature secreted form of amphiregulin is an 84-amino acid residue glycosylated polypeptide which is generated by proteolytic processing of a 252-amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR; these are EGF, TGFa, AR, HB-EGF, cripto-1, epiregulin, and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. Amphiregulin expression is induced by phorbol ester, estrogen, androgen, and other EGFR ligands. In vitro, amphiregulin functions as an autocrine growth stimulator to drive proliferation of colon carcinoma cells, normal and oncogene-transformed mammary epithelial cells, cervical carcinoma cells, prostate cancer cells, and keratinocytes. Amphiregulin is important in the development of human placenta and murine mammary gland. It is reportedly overexpressed in human cancers of breast, colon, stomach and pancreas.
- A REG
ab99975 被引用在 6 文献中.
- Luo M & Luo Y Imperatorin Relieved Ulcerative Colitis by Regulating the Nrf-2/ARE/HO-1 Pathway in Rats. Inflammation 44:558-569 (2021). PubMed: 33098052
- Naffar-Abu Amara S et al. Transient commensal clonal interactions can drive tumor metastasis. Nat Commun 11:5799 (2020). PubMed: 33199705
- Han LL et al. miR-103 promotes the metastasis and EMT of hepatocellular carcinoma by directly inhibiting LATS2. Int J Oncol 53:2433-2444 (2018). PubMed: 30272278
- Boire A et al. Complement Component 3 Adapts the Cerebrospinal Fluid for Leptomeningeal Metastasis. Cell 168:1101-1113.e13 (2017). PubMed: 28283064
- Barton VN et al. Multiple molecular subtypes of triple-negative breast cancer critically rely on androgen receptor and respond to enzalutamide in vivo. Mol Cancer Ther 14:769-78 (2015). PubMed: 25713333
- Hutchinson KE et al. ERBB activation modulates sensitivity to MEK1/2 inhibition in a subset of driver-negative melanoma. Oncotarget 6:22348-60 (2015). PubMed: 26084293