人Albumin ELISA试剂盒(ab227933)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 30 pg/ml
- Range: 312.5 pg/ml - 20000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人Albumin ELISA试剂盒
参阅全部 Albumin 试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% Serum 9 4% 批次间 样品 n Mean SD CV% Serum 3 12.6% -
样品类型
Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
检测类型
Sandwich (quantitative) -
灵敏度
30 pg/ml -
范围
312.5 pg/ml - 20000 pg/ml -
回收率
特定样本回收率 样品类型 平均% 范围 Urine 104 101% - 107% Serum 117 106% - 133% Hep Plasma 111 104% - 116% EDTA Plasma 115 108% - 121% Cit plasma 112 106% - 117% -
检测时间
1h 30m -
实验步骤
One step assay -
种属反应性
与反应: Human
不与反应: Cow, Rhesus monkey -
产品概述
Human Albumin ELISA Kit (ab227933) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Albumin protein in cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Albumin with 30 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
CROSS REACTIVITY Purified mouse, bovine, and cynomolgus monkey serum albumin were prepared at 1,000,1000, and 500 ng/mL, respectively, and assayed for cross reactivity. No cross-reactivity was observed.
SPECIES REACTIVITY This kit recognizes Human Albumin protein. Other species reactivity was determined by measuring 1:5 million serum or plasma samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum or plasma assayed at the same dilution. Reactivity < 3% was determined for the following species: Mouse, Rat, Cow and Rhesus Macaque -
平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 x 96 tests 1 x 384 tests 1 x 96 tests 10X Human Albumin Capture Antibody 1 x 600µl 1 x 600µl 1 x 600µl 10X Human Albumin Detector Antibody 1 x 600µl 1 x 600µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 2 x 20ml 1 x 20ml 384 well CaptSure™ microplates 0 x 0 unit 1 unit 0 x 0 unit Antibody Diluent CP2 1 x 6ml 1 x 6ml 1 x 6ml Native Human Serum Albumin protein (Lyophilized) (ab205808) 2 vials 2 vials 2 vials Plate Seals 1 unit 1 unit 1 unit Sample Diluent NS (ab193972) 1 x 50ml 1 x 250ml 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 0 x 0 unit 1 unit Stop Solution 1 x 12ml 2 x 12ml 1 x 12ml TMB Development Solution 1 x 12ml 2 x 12ml 1 x 12ml -
研究领域
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功能
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
组织特异性
Plasma. -
疾病相关
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
序列相似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
翻译后修饰
Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
细胞定位
Secreted. - Information by UniProt
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别名
- alb
- ALBU_HUMAN
- Albumin (32 AA)
see all -
数据库链接
- Entrez Gene: 213 Human
- Omim: 103600 Human
- SwissProt: P02768 Human
- Unigene: 418167 Human
- Unigene: 592379 Human
相关产品
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Alternative Versions
图片
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Background-subtracted data values (mean +/- SD) are graphed.
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Example of human Albumin standard curve in Sample Diluent NS. The Albumin standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of Serum Albumin were measured in duplicates, interpolated from the Serum Albumin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:5 million, plasma (citrate) 1:5 million, plasma (heparin) 1:5 million, and plasma (EDTA) 1:10 million. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serum Albumin concentration was determined to be 39.3 mg/mL in serum, 34.4 mg/mL in plasma (citrate), 30.8 mg/mL in plasma (heparin), and 34.5 mg/mL in plasma (EDTA).
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The concentrations of Serum Albumin were measured in duplicates, interpolated from the Serum Albumin standard curves and corrected for sample dilution. Undiluted samples are 1:500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serum Albumin concentration was determined to be 6,290 ng/mL in urine.
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Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serum Albumin concentration was determined to be 33.31 mg/mL with a range of 22.5 – 46.2 mg/mL.
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Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native Albumin was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab227933 被引用在 2 文献中.
- Coppens E et al. Gemcitabine lipid prodrug nanoparticles: Switching the lipid moiety and changing the fate in the bloodstream. Int J Pharm 609:121076 (2021). PubMed: 34481886
- Kim TH et al. Removal of large middle molecules via haemodialysis with medium cut-off membranes at lower blood flow rates: an observational prospective study. BMC Nephrol 21:2 (2019). PubMed: 31892319