人MS2 ELISA试剂盒(ab213750)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
概述
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产品名称
人MS2 ELISA试剂盒 -
检测方法
Colorimetric -
精确度
批次内 样品 n Mean SD CV% 1 16 0.22ng/ml 0.013 5.7% 2 16 1.47ng/ml 0.09 6.1% 3 16 2.34ng/ml 0.185 7.9% 批次间 样品 n Mean SD CV% 1 24 0.27ng/ml 0.018 6.8% 2 24 1.56ng/ml 0.115 7.4% 3 24 2.58ng/ml 0.214 8.3% -
样品类型
Cell culture supernatant, Serum -
检测类型
Sandwich (quantitative) -
灵敏度
< 10 pg/ml -
范围
62.5 pg/ml - 4000 pg/ml -
检测时间
3h 30m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Human -
产品概述
The Human MS2 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213750) is designed for the quantitative measurement of Human MS2 in cell culture supernatants and serum.
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for MS2 has been pre-coated onto 96-well plates. Standards (NSO, M1-P497) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MS2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human MS2 amount of sample captured in plate.
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说明
MS2 (also known as ADAM8), a disintegrin and metalloproteinase domain-containing protein 8 is an enzyme that in humans is encoded by the MS2 /ADAM8 gene. MS2 is localized to chromosome 10q26.3. This gene encodes a member of the ADAM (a disintegrin and metalloproteinase domain) family. Members of the ADAM family, such as MS2, are cell surface proteases involved in remodeling of extracellular matrix, cell migration, and processing of membrane-bound signaling molecules. They are characterized by disintegrin and metalloprotease domains that confer adhesive properties and proteolytic activities, respectively. The protein encoded by this gene may be involved in cell adhesion during neurodegeneration.
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平台
Pre-coated microplate (12 x 8 well strips)
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 标识符 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human MS2 coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti- Human MS2 antibody 1 x 100µl Lyophilized recombinant Human MS2 standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
研究领域
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功能
Possible involvement in extravasation of leukocytes. -
组织特异性
Expressed on neutrophils and monocytes. -
序列相似性
Contains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain. -
细胞定位
Membrane. - Information by UniProt
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别名
- A Disintegrin And Metalloproteinase domain 8
- ADAM 8
- ADAM 8 precursor
see all -
数据库链接
- Entrez Gene: 101 Human
- Omim: 602267 Human
- SwissProt: P78325 Human
- Unigene: 501574 Human
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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