Anti-Hub1抗体(ab4754)

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Western blot - Anti-Hub1 antibody (ab4754)

    Key features and details

    • Rabbit polyclonal to Hub1
    • Suitable for: WB
    • Reacts with: Saccharomyces cerevisiae
    • Isotype: IgG

    概述

    • 产品名称

      Anti-Hub1抗体

    • 描述

      兔多克隆抗体to Hub1

    • 宿主

      Rabbit

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Saccharomyces cerevisiae

    • 免疫原

      Recombinant full length protein (S. cerevisiae).

    • 阳性对照

      • Most yeast cell lysates can be used as a positive control without induction or stimulation.

    • 常规说明

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • 存储溶液

      Preservative: 0.01% Sodium azide
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
    • Concentration information loading...

    • 纯度

      IgG fraction

    • 纯化说明

      Purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum.

    • 克隆

      多克隆

    • 同种型

      IgG

    • 研究领域

    应用

    Our Abpromise guarantee covers the use of ab4754 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB 1/500. Detects a band of approximately 9.7 kDa.

    靶标

    • 相关性

      Hub1 may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog.

    • 数据库链接

      • 别名

        • Hub1 protein antibody
        • HUB1 target protein 1 antibody
        • Hub1p antibody
        • Ubiquitin like protein modifier antibody

      图片

      • Western blot - Anti-Hub1 antibody (ab4754)
        Western blot - Anti-Hub1 antibody (ab4754)
        ab4754 was tested by immunoblot against yeast lysates expressing the Hub1-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL.   Panel A shows total protein staining using ponceau.  Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1/1000 dilution of Goat polyclonal to GFP (ab6673) followed by reaction with a 1/15000 dilution of Donkey polyclonal to Goat IgG (HRP)(ab7125).  Panel C shows specific reaction with Hub1 using a 1/500 dilution of ab4754 followed by reaction with a 1/15000 dilution of Goat polyclonal to Rabbit IgG (HRP) (ab7090).  All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4° C.  Yeast lysate proteins were separated by S

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