Key features and details
- Rabbit polyclonal to Hub1
- Suitable for: WB, ELISA
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
经测试应用适用于: WB, ELISAmore details
种属反应性与反应: Saccharomyces cerevisiae
Recombinant full length protein (S. cerevisiae).
- Most yeast cell lysates can be used as a positive control without induction or stimulation.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
纯化说明Purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum.
Our Abpromise guarantee covers the use of ab4754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 9.7 kDa.|
|ELISA||1/1000 - 1/5000.|
相关性Hub1 may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog.
- Hub1 protein antibody
- HUB1 target protein 1 antibody
- Hub1p antibody
- Ubiquitin like protein modifier antibody
ab4754 was tested by immunoblot against yeast lysates expressing the Hub1-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1/1000 dilution of Goat polyclonal to GFP (ab6673) followed by reaction with a 1/15000 dilution of Donkey polyclonal to Goat IgG (HRP)(ab7125). Panel C shows specific reaction with Hub1 using a 1/500 dilution of ab4754 followed by reaction with a 1/15000 dilution of Goat polyclonal to Rabbit IgG (HRP) (ab7090). All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4° C. Yeast lysate proteins were separated by S