Anti-HSV1 ICP0抗体[5H7] (ab6513)

Reviews (1)Q&A (1)References (27)

Key features and details

  • Mouse monoclonal [5H7] to HSV1 ICP0
  • Suitable for: WB, ELISA, ICC/IF
  • Isotype: IgG1

概述

  • 产品名称

    Anti-HSV1 ICP0抗体[5H7]

  • 描述

    小鼠单克隆抗体[5H7] to HSV1 ICP0

  • 宿主

    Mouse

  • 经测试应用

    适用于: WB, ELISA, ICC/IFmore details

  • 种属反应性

    与反应: Other species

  • 免疫原

    Herpes Virus

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • 存储溶液

    pH: 7.4
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    5H7

  • 骨髓瘤

    NS1/1-Ag4-1

  • 同种型

    IgG1

  • 轻链类型

    kappa

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/8000.
ELISA 1/12800.
ICC/IF 1/25600.

靶标

  • 相关性

    Herpes simplex virus (HSV) encodes four nuclear phosphoproteins that contribute towards the full productive lytic cycle cascade in newly infected permissive cells. The IE175 (or ICP4) and IE63 (or ICP27) proteins have essential regulatory roles during the lytic cycle. A third, IE110 (or ICP0), appears to play only an accessory role in the presence of VP16, but in the absence of VP16 appears to be critical for initiation of reactivated infections.

  • 细胞定位

    Nuclear

  • 别名

    • Alpha 0 protein antibody
    • Herpes siplex virus 1 antibody
    • HHV1 ICP0 antibody
    • HSV1 IE110 antibody
    • Human Herpesvirus 1 antibody
    • IE110 antibody
    • Immediate early nuclear phosphoprotein antibody
    • Immediate early protein IE110 antibody
    • Trans acting transcriptional protein ICP0 antibody
    • VMW110 antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP0 antibody [5H7] (ab6513)
    Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP0 antibody [5H7] (ab6513)This image is courtesy of an anonymous Abreview
    ab6513 (1/25600) staining HSV1 ICP0 in human cells. Cells were PFA-fixed, permeabilized in 0.3% TritonX-100/PBS and blocked in 1% serum /30 minutes /25oC. A fluorescently labeled donkey anti-mouse antibody was used as the secondary.

    See Abreview

文献 (27)

发表研究结果有使用 ab6513？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab6513 被引用在 27 文献中.

  • Furey C  et al. TACC3 Regulates Microtubule Plus-End Dynamics and Cargo Transport in Interphase Cells. Cell Rep 30:269-283.e6 (2020). PubMed: 31914393
  • Subramanian G  et al. The interferon-inducible protein TDRD7 inhibits AMP-activated protein kinase and thereby restricts autophagy-independent virus replication. J Biol Chem 295:6811-6822 (2020). PubMed: 32273341
  • Wang Z  et al. MiR-155-5p modulates HSV-1 replication via the epigenetic regulation of SRSF2 gene expression. Epigenetics 14:494-503 (2019). PubMed: 30950329
  • Li F  et al. Amentoflavone Inhibits HSV-1 and ACV-Resistant Strain Infection by Suppressing Viral Early Infection. Viruses 11:N/A (2019). PubMed: 31121928
  • Yan X  et al. miR-138 expression in oral herpes simplex and its effect on ICP0. Exp Ther Med 17:388-392 (2019). PubMed: 30651809
View all Publications for this product

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Immunocytochemistry/ Immunofluorescence abreview for Anti-HSV1 ICP0 antibody [5H7]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HSV1 infected or transfected with plasmid)
Specification
HSV1 infected or transfected with plasmid
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% TritonX-100 in PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Oct 12 2007

Question

Could you let me know if this antibody can be used to stain fixed tissue sections. If not, do you have any antibodies available to HSV1 ICP0 or ICP5 that can be used to immunohistochemically stain slides?

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Answer

According to the datasheet, this antibody has not been tested for immunohistochemistry. I have looked through our catalogue and am very sorry to inform you that we currently do not stock products that suit to your requirement. If you cannot find what you are looking for within the Abcam catalog, I suggest that you click on the link to The World's Antibody Gateway. The World's Antibody Gateway is a free search engine service provided by Abcam to help you to quickly find the antibodies that you are looking for. It is a free-text search engine developed by the Abcam team so that it searches the catalogs of all online antibody companies (currently 249).

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