The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC - Wholemount
Use at an assay dependent concentration. PubMed: 21998580
Use at an assay dependent concentration.
This antibody may be diluted to a titer of 1:75-1:200 in an ABC method. We suggest an incubation period of 30-60 minutes at room temperature. Pretreat with citrate buffer pH 6.0.
Use at an assay dependent concentration. PubMed: 18042934
Use at an assay dependent concentration. PubMed: 27783096
Use at an assay dependent concentration. PubMed: 22003387
Herpes simplex type 1 (HSV-1) belongs to a family that includes HSV-2, Epstein-Barr virus (EBV) and Varicella zoster (chicken pox) virus amongst others. HSV-1 and HSV-2 are extremely difficult to distinguish from each other. Members of this family have a characteristic virion structure. The double stranded DNA genome is contained within an icosahedral capsid embedded in a proteinaceous layer (tegument) and surrounded by a lipid envelope, derived from the nuclear membrane of the last host, which is decorated with virus-specific glycoproteins spikes. These viruses are capable of entering a latent phase where the host shows no visible sign of infection and levels of infectious agent become very low.
Formalin-fixed, paraffin-embedded HSV1-infected human tissue stained for HSV1 with ab9533 in immunohistochemical analysis.
IHC - Wholemount - Anti-HSV1 antibody (ab9533)Image from Wuest T et al., PLoS Pathog. 2011 Oct;7(10):e1002278. Epub 2011 Oct 6. Fig 1.; doi:10.1371/journal.ppat.1002278; October 6, 2011, PLoS Pathog 7(10): e1002278.
ab9533, staining HSV1 in cornea from pVEGFA-GFP reporter mice at day 3 post-infection with HSV1 strain McKrae.
Corneas were fixed in 4% paraformaldehyde, and washed with PBS containing 1% Triton X-100 before being stained with primary antibody. A Dylight®549-conjugated donkey anti-rabbit IgG was used as the secondary antibody.