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Microbiology Organism Virus DNA Virus double stranded DNA Virus Herpes simplex

Anti-HSV1 + HSV2 ICP5 Major Capsid蛋白抗体[3B6] - BSA and Azide free (ab6508)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (41)

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Immunocytochemistry/ Immunofluorescence - Anti-HSV1 + HSV2 ICP5 Major Capsid Protein antibody [3B6] - BSA and Azide free (ab6508)

    Key features and details

    • Mouse monoclonal [3B6] to HSV1 + HSV2 ICP5 Major Capsid Protein - BSA and Azide free
    • Suitable for: IHC-P, WB, ELISA, ICC/IF
    • Isotype: IgG2b

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    概述

    • 产品名称

      Anti-HSV1 + HSV2 ICP5 Major Capsid蛋白抗体[3B6] - BSA and Azide free
    • 描述

      小鼠单克隆抗体[3B6] to HSV1 + HSV2 ICP5 Major Capsid蛋白- BSA and Azide free
    • 宿主

      Mouse
    • 经测试应用

      适用于: IHC-P, WB, ELISA, ICC/IFmore details
    • 种属反应性

      与反应: Other species
    • 免疫原

      Tissue, cells or virus corresponding to HSV1 + HSV2 ICP5 Major Capsid Protein.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.40
      Constituent: PBS
    • 无载体

      是
    • Concentration information loading...
    • 纯度

      Protein A purified
    • 克隆

      单克隆
    • 克隆编号

      3B6
    • 骨髓瘤

      NS1/1-Ag4-1
    • 同种型

      IgG2b
    • 轻链类型

      kappa
    • 研究领域

      • Microbiology
      • Organism
      • Virus
      • DNA Virus
      • double stranded DNA Virus
      • Herpes simplex

    相关产品

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab6508于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P (1)
    Use at an assay dependent concentration.
    WB
    Use at an assay dependent concentration.

    Western Blot Titer: HSV-1 >1:3,200; HSV-2 >1:3,200.

    ELISA
    Use at an assay dependent concentration.

    ELISA titer: HSV-1 0.100 @ >1:102,400; HSV-2 0.100 @ >1:102,400.

    ICC/IF
    Use at an assay dependent concentration. PubMed: 18701599

    IFA titer: HSV-1 1+ @ 1:3,200; HSV-2 1+ @ 1:3,200.

    说明
    IHC-P
    Use at an assay dependent concentration.
    WB
    Use at an assay dependent concentration.

    Western Blot Titer: HSV-1 >1:3,200; HSV-2 >1:3,200.

    ELISA
    Use at an assay dependent concentration.

    ELISA titer: HSV-1 0.100 @ >1:102,400; HSV-2 0.100 @ >1:102,400.

    ICC/IF
    Use at an assay dependent concentration. PubMed: 18701599

    IFA titer: HSV-1 1+ @ 1:3,200; HSV-2 1+ @ 1:3,200.

    靶标

    • 相关性

      Herpes simplex virus 1 and 2 (HSV-1 and HSV-2) are two species of the herpes virus family, Herpesviridae, which cause infections in humans. They are also called Human Herpes Virus 1 and 2 (HHV-1 and HHV-2) and are neurotropic and neuroinvasive viruses; they enter and hide in the human nervous system, accounting for their durability in the human body.
    • 别名

      • Capsid protein VP5 antibody
      • Herpes simplex virus 1 major capsid protein antibody
      • Herpes simplex virus 2 major capsid protein antibody
      • Human Herpes virus antibody
      • Major capsid protein antibody
      • MCP antibody
      • UL19 antibody
      • VP5 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-HSV1 + HSV2 ICP5 Major Capsid Protein antibody [3B6] - BSA and Azide free (ab6508)
      Immunocytochemistry/ Immunofluorescence - Anti-HSV1 + HSV2 ICP5 Major Capsid Protein antibody [3B6] - BSA and Azide free (ab6508)Image from Ohta A et al., Virol J. 2011 Jul 26;8:365. doi: 10.1186/1743-422X-8-365.; Fig 4.; 26 July 2011, Virology Journal 2011, 8:365
      Immunofluorescence analysis of HSV infected cells, 7 hours (left) or 17 hours (right) post-infection, staining HSV1 + HSV2 ICP5 Major Capsid Protein with ab6508 at 1/200 dilution.

      Cells were fixed for 10 min in paraformaldehyde, permeabilized in 0.1% Triton X-100 and blocked in 4% goat serum, 1% bovine serum albumin, before incubating with primary antibody for 30 min at room temperature. An AlexaFluor®647-conjugated goat anti-mouse IgG1 was used as the secondary antibody.

    实验方案

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (41)

    发表研究结果有使用 ab6508?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6508 被引用在 41 文献中.

    • Wang Y  et al. Single-cell RNA-sequencing analysis identifies host long noncoding RNA MAMDC2-AS1 as a co-factor for HSV-1 nuclear transport. Int J Biol Sci 16:1586-1603 (2020). PubMed: 32226304
    • Chen Y  et al. Single AAV-Mediated CRISPR-SaCas9 Inhibits HSV-1 Replication by Editing ICP4 in Trigeminal Ganglion Neurons. Mol Ther Methods Clin Dev 18:33-43 (2020). PubMed: 32577430
    • Wang W  et al. Guanidine modifications enhance the anti-herpes simplex virus activity of (E,E)-4,6-bis(styryl)-pyrimidine derivatives in vitro and in vivo. Br J Pharmacol 177:1568-1588 (2020). PubMed: 31709511
    • Harancher MR  et al. Antiviral Properties of the LSD1 Inhibitor SP-2509. J Virol 94:N/A (2020). PubMed: 32699090
    • Hait AS  et al. Defects in LC3B2 and ATG4A underlie HSV2 meningitis and reveal a critical role for autophagy in antiviral defense in humans. Sci Immunol 5:N/A (2020). PubMed: 33310865
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HSV1 + HSV2 ICP5 Major Capsid Protein antibody [3B6]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Head and neck cancer, mouse xenograft)
    Specification
    Head and neck cancer, mouse xenograft
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate, pH=6
    Permeabilization
    No
    Blocking step
    Vector Lab Mouse Ig Blocking Reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 23°C
    Read More

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    提交于 May 14 2013

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