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Microbiology Organism Virus DNA Virus double stranded DNA Virus Herpes simplex

Anti-HSV1 + HSV2 gB抗体[10B7] (ab6506)

  • Datasheet
  • SDS
Submit a review Q&A (10)References (15)

Product price, shipping and contact information

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Abpromise

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Immunocytochemistry/ Immunofluorescence - Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506)
  • Western blot - Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506)

Key features and details

  • Mouse monoclonal [10B7] to HSV1 + HSV2 gB
  • Suitable for: WB, ELISA, ICC/IF
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-HSV1 + HSV2 gB抗体[10B7]
  • 描述

    小鼠单克隆抗体[10B7] to HSV1 + HSV2 gB
  • 宿主

    Mouse
  • 经测试应用

    适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性

    与反应: Other species
  • 免疫原

    Herpes Virus

  • 阳性对照

    • African Green Monkey Kidney cultured cells(Vero), HSV-1 is strain MacIntyre, HSV-2 is strain G.
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.4
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    10B7
  • 骨髓瘤

    NS1/1-Ag4-1
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • double stranded DNA Virus
    • Herpes simplex

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

应用

Our Abpromise guarantee covers the use of ab6506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.

1/1600 (HSV2) - 1/12800 (HSV1).

ELISA 1/102400.
ICC/IF Use at an assay dependent concentration. 1/6400 (HSV1) - 1/25600 (HSV2).

靶标

  • 相关性

    There are two main types of Herpes Simplex Virus (HSV), 1 and 2. The primary difference between the two types is in where they typically establish latency in the body. HSV1 usually establishes latency in the trigeminal ganglion, a collection of nerve cells near the ear. From there, it tends to recur on the lower lip or face causing cold sores. HSV2 usually resides in the sacral ganglion at the base of the spine. From there, it recurs in the genital area but even this difference is not absolute as in rare cases it can infect any part of the body, including the eyes. Up to 30% of genital herpes are also caused by HSV1. Both HSV1 and 2 are highly contagious through contact.
  • 细胞定位

    Virus envelope
  • 别名

    • Envelope glycoprotein B antibody
    • GB antibody
    • Glycoprotein B antibody
    • HSV1 antibody
    • HSV2 antibody
    • UL27 antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506)
    Immunocytochemistry/ Immunofluorescence - Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506)
    Green: immunofluorescence staining of HSV1 or HSV2 of Vero (African green monkey) cells ( HSV-1 is strain MacIntyre, HSV-2 is strain G) on slides, using ab6506 (1:100) (secondary anti-mouse-FITC). Red: counterstain in the conjugate preparation.
  • Western blot - Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506)
    Western blot - Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506)
    Lane 1 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/12800 dilution
    Lane 2 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/1600 dilution

    Lane 1 : Vero cells infected with HSV1
    Lane 2 : Vero cells infected with HSV2

    Performed under reducing conditions.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (15)

    发表研究结果有使用 ab6506?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab6506 被引用在 15 文献中.

    • Sato E  et al. Loss of ATP2A2 Allows Herpes Simplex Virus 1 Infection of a Human Epidermis Model by Disrupting Innate Immunity and Barrier Function. J Invest Dermatol N/A:N/A (2018). PubMed: 29870688
    • Liu Y  et al. HSV-2 glycoprotein J promotes viral protein expression and virus spread. Virology 525:83-95 (2018). PubMed: 30248525
    • Wang Y  et al. Heat-shock protein 90a is involved in maintaining the stability of VP16 and VP16-mediated transactivation of a genes from herpes simplex virus-1. Mol Med 24:65 (2018). PubMed: 30577726
    • Zhang M  et al. Herpes Simplex Virus Type 2 Infection-Induced Expression of CXCR3 Ligands Promotes CD4+ T Cell Migration and Is Regulated by the Viral Immediate-Early Protein ICP4. Front Immunol 9:2932 (2018). PubMed: 30619292
    • Merkl PE  et al. Mechanisms of Host IFI16, PML, and Daxx Protein Restriction of Herpes Simplex Virus 1 Replication. J Virol 92:N/A (2018). PubMed: 29491153
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 10 Abreviews or Q&A

    Question

    What is the expected band size?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 22 2012

    Answer

    Thank you for contacting us.

    The following article will be helpful for further reading; http://www.ncbi.nlm.nih.gov/pubmed/2475970

    We can provide the blocking peptide , which was used for producing this antibody? Could you confirm if you are happy purchasing this and then I will send you the price?

    I have checked the WB protocol we used to test this antibody. This protocol is no different than the any general protocol. We recommend using reduced and denaturing conditions.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    回复于 Nov 22 2012

    Question

    I have decided to buy the mouse monoclonal antibody directed against HSV-1 gB ab6506. Do you know how long woud it take for the antibody to arrive to Bogotá, Colombia (South America) once I order it?.    I am interested in the promo code you offered me, So I wait for your e-mail with the code and then I buy the antibody, Right?. 

    Read More

    Abcam community

    Verified customer

    Asked on Apr 30 2012

    Answer

    I am very pleased to hear you would like to accept our offer and test ab6506 in ICC on neurons. This code will give you: 1 free PRIMARY ANTIBODY OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for this application and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. All orders to Colombia require pre-payment. You can pay by credit card, or our customer service group can send you a proforma invoice which you will need to pay before the goods are shipped - this can be requested when you place an order. Our customer service representatives will also be able to provide you with further delivery information at that time. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    回复于 Apr 30 2012

    Question

    Inquiry: Hello I would like to know if these monoclonal mouse antibodies ab0656 and ab6509 directed against HSV-1 gB and HSV-1 gC, Have been used to detect infected neurons in culture. Or If they can work to identify Infected neurons in histological preparations?. And what kind of fixation substance (PFA or Methanol) shoul I use to fix my neuron cells?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 28 2012

    Answer

    Thank you very much for your interest in ab6506 and ab6509.

    To our knowledge, these products have not been tested inneurons in culture. Therefore, I can offer a discount off a future purchase if you buy one or both of these products now, test it in ICC on neurons and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free PRIMARY ANTIBODY OR VALUE OFF OF NEXT ORDER for each antibody you test

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test one or both of these products in ICC on neurons. I will then send a discount code. These codes must be issued before purchasing so please wait for my reply before ordering.

    2. Purchase either by phone, fax, or online (www.abcam.com).

    3. Test it in neurons.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. Each discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the product turns out to be unsuitable for this application, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    回复于 Apr 28 2012

    Question

    For Western Blot Analysis I used your Anti-HSV1 + HSV2 gB antibody [10B7]. I had no problems with this antibody, when I worked with the wild type gB (HSV-1). But the antibody does not work with c-terminal deletion mutants of the viral protein. I would like to know which gB epitopes the antibody recognizes, but can not find any information on
    your website. Is it possible to get more information about this antibody??

    Read More

    Abcam community

    Verified customer

    Asked on Mar 02 2012

    Answer

    Thank you for your enquiry.

    The immunogen used to generate the antibody clone [10B7] (ab6506) was the entire Herpes Virus. Unfortunately, the exact epitope recognised by this monoclonal antibody has not been mapped.

    To our knowledge this antibody has been shown to have virus neutralizing activity, and thus it is likely that the antibody binds an extracellular domain. We unfortunately do not have data on this available though.

    Maybe a search in the literature could help to find more details about this clone. The publication by Reszka and colleagues (PMID 20117270, attached) might be a good starting point.

    We aim to provide as much information as possible to customers, so I am sorry that this has not been possible on this occasion. Should you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    回复于 Mar 02 2012

    Question

    Dear Kate,

    Here is the order number:
    Abcamorder (ref:ORDER ####### confirmation [CCE3400448]

    Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Feb 09 2012

    Answer

    Thank you for confirming these details and for your cooperation.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued an alternative free of charge replacement of ab8232:

    Order number: ######

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    回复于 Feb 09 2012

    Question

    I have finished and done two western blot experiments as well as two IF experiments, both with different concentration of antibody and got no results.

    The western blot, I tried 2 times with different dilutions of antibody as well as free virus in the last blot which only showed the ladder and I've attached to this email.

    I'm including the second set of IF I did and still no band just background was seen with the green channel. The second antibody 488 works well and is used often in the lab with other primary antibodies. The only thing you can see is the dapi stain.

    I would really appreciate if you could send us another HSV-1 antibody that you know works well, specifically has nice images in Immunofluorescence. I think 3 weeks of trying out theAb6506antibody is enough time.

    Thank you and hope to hear from you soon.

    Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Feb 07 2012

    Answer

    Thank you for your message and for providing this further information.

    I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased to arrange a free of charge replacement or credit note in compensation.

    I would like to reassure you that we monitor feedback closely on a weekly basis and we are not currently concerned about the general quality of this antibody or this batch. Regrettably, I can suggest you have received a bad vial on this occasion.

    Therefore, we would be pleased to provide a replacement of the same antibody, which will still be covered by our guarantee. Alternatively, I fully understand the your concerns and if you prefer an alternative replacement or credit note in this case, I will be pleased to arrange this for you.

    We have one other i-HSV1 + HSV2 gB antibody which has been testd in ICC-IF and has ICC-IF image on the datasheet:

    HSV1 + HSV2 gB antibody [T111] (ab8232)
    https://www.abcam.com/index.html?datasheet=8232 https://www.abcam.com/index.html?
    datasheet=8232.

    I hope this will be helpful. I look forward to hearing from you with details of how you would like to proceed.

    Read More

    Abcam Scientific Support

    回复于 Feb 07 2012

    Question

    I'm looking for an antibody to use in sELISA with a monoclonal to the N-terminal. What is the immunogen/epitope?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 06 2012

    Answer

    Thank you for your inquiry. I heard back from the lab and unfortunately neither ab6506 nor ab33040 have been epitope mapped. We do not know if these antibodies will work in your sELISA since we do not know where the antibodies are binding. I am sorry that this information could not be more helpful. Please contact us with any other questions. Our scientist sent us the following reply to your inquiry. It is as follows: We do not have epitope information. In some cases, you can find a literature reference on the webpage that describes the antibody. This literature may have the epitope information. For pairing experiments, it may be most effective to just try it.

    Read More

    Abcam Scientific Support

    回复于 Jan 06 2012

    Question

    Hi. Can you tell me, whether this antibody bids to the extracellular domain of the gB protein and where it binds (optimal would be to know epitope/region). Thanks in advance Best Regards

    Read More

    Abcam community

    Verified customer

    Asked on Jul 14 2006

    Answer

    Thank you for your enquiry. I have obtained some details on this antibody for you. As it is a virus neutralising antibody, it is highly likely that it binds to the extracellular domain. However, there is no information available on the precise epitope/region of the protein that this antibody is raised against. I hope this helps. Please get back to me if you require more help. With thanks.

    Read More

    Abcam Scientific Support

    回复于 Jul 17 2006

    Question

    Customer reported receiving 60 ul rather than 100 ul in her vial of ab6506. Abcam lot# 104235. Order# 81204.

    Read More

    Abcam community

    Verified customer

    Asked on May 02 2005

    Answer

    Thank you for your phone call. I apologize for the shortage that you have received and a replacement is being sent to you free of charge on order# 81816 which you should receive in approximately one week. I did contact the source of this product and they said that sometimes the antibodies slowly lose water under normal storage conditions. You are not short of protein, just volume (water), and they suggested adding 43 ul of deionized water to the vial that you received. However, I am still sending you a replacement vial. Please let me know if you have any additional questions.

    Read More

    Abcam Scientific Support

    回复于 May 03 2005

    Question

    Does this antibody detect glycoprotein B in both HSV-I and HSV-II? Thank you, Joel Conrad

    Read More

    Abcam community

    Verified customer

    Asked on Jul 18 2001

    Answer

    Hi Joel, Yes it does detect gB from both type 1 and type 2 HSV. Please see our testing data at: HSV gB Mab

    Read More

    Abcam Scientific Support

    回复于 Jul 18 2001

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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