Anti-HSV1 + HSV2 gB抗体[10B7] (ab6506)
Key features and details
- Mouse monoclonal [10B7] to HSV1 + HSV2 gB
- Suitable for: WB, ELISA, ICC/IF
- Isotype: IgG1
概述
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产品名称
Anti-HSV1 + HSV2 gB抗体[10B7] -
描述
小鼠单克隆抗体[10B7] to HSV1 + HSV2 gB -
宿主
Mouse -
经测试应用
适用于: WB, ELISA, ICC/IFmore details -
种属反应性
与反应: Other species -
免疫原
Tissue, cells or virus corresponding to HSV1 + HSV2 gB.
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阳性对照
- African Green Monkey Kidney cultured cells(Vero), HSV-1 is strain MacIntyre, HSV-2 is strain G.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.4 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
10B7 -
骨髓瘤
NS1/1-Ag4-1 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use at an assay dependent concentration.
1/1600 (HSV2) - 1/12800 (HSV1). |
|
ELISA |
1/102400.
|
|
ICC/IF |
Use at an assay dependent concentration. 1/6400 (HSV1) - 1/25600 (HSV2).
|
说明 |
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WB
Use at an assay dependent concentration. 1/1600 (HSV2) - 1/12800 (HSV1). |
ELISA
1/102400. |
ICC/IF
Use at an assay dependent concentration. 1/6400 (HSV1) - 1/25600 (HSV2). |
靶标
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相关性
There are two main types of Herpes Simplex Virus (HSV), 1 and 2. The primary difference between the two types is in where they typically establish latency in the body. HSV1 usually establishes latency in the trigeminal ganglion, a collection of nerve cells near the ear. From there, it tends to recur on the lower lip or face causing cold sores. HSV2 usually resides in the sacral ganglion at the base of the spine. From there, it recurs in the genital area but even this difference is not absolute as in rare cases it can infect any part of the body, including the eyes. Up to 30% of genital herpes are also caused by HSV1. Both HSV1 and 2 are highly contagious through contact. -
细胞定位
Virus envelope -
别名
- Envelope glycoprotein B antibody
- GB antibody
- Glycoprotein B antibody
see all
图片
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Green: immunofluorescence staining of HSV1 or HSV2 of Vero (African green monkey) cells ( HSV-1 is strain MacIntyre, HSV-2 is strain G) on slides, using ab6506 (1:100) (secondary anti-mouse-FITC). Red: counterstain in the conjugate preparation.
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Lanes 1 & 8 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/1600 dilution
Lane 2 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/3200 dilution
Lane 3 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/6400 dilution
Lane 4 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/12800 dilution
Lane 5 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/200 dilution
Lane 6 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/400 dilution
Lane 7 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/800 dilution
Lanes 1-4 : Vero cells infected with HSV1
Lanes 5-8 : Vero cells infected with HSV2
Performed under reducing conditions.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (26)
ab6506 被引用在 26 文献中.
- Mertens ME & Knipe DM Herpes Simplex Virus 1 Manipulates Host Cell Antiviral and Proviral DNA Damage Responses. mBio 12:N/A (2021). PubMed: 33563816
- Gong Y et al. High-efficiency nonhomologous insertion of a foreign gene into the herpes simplex virus genome. J Gen Virol 101:982-996 (2020). PubMed: 32602833
- Li W et al. Autophagic degradation of PML promotes susceptibility to HSV-1 by stress-induced corticosterone. Theranostics 10:9032-9049 (2020). PubMed: 32802177
- Zan J et al. RNA helicase DDX5 suppresses IFN-I antiviral innate immune response by interacting with PP2A-Cß to deactivate IRF3. Exp Cell Res 396:112332 (2020). PubMed: 33065113
- Wang W et al. Guanidine modifications enhance the anti-herpes simplex virus activity of (E,E)-4,6-bis(styryl)-pyrimidine derivatives in vitro and in vivo. Br J Pharmacol 177:1568-1588 (2020). PubMed: 31709511