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Microbiology Organism Virus DNA Virus double stranded DNA Virus Herpes simplex

抗单纯疱疹病毒1 ICP4 Immediate Early蛋白抗体[10F1] (ab6514)

  • Datasheet
Reviews (5) Submit a question References (41)

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Western blot - Anti-HSV 1 ICP4 Immediate Early Protein  antibody [10F1] (ab6514)
  • Western blot - Anti-HSV 1 ICP4 Immediate Early Protein  antibody [10F1] (ab6514)
  • Immunocytochemistry/ Immunofluorescence - Anti-HSV 1 ICP4 Immediate Early Protein  antibody [10F1] (ab6514)

Key features and details

  • Mouse monoclonal [10F1] to HSV 1 ICP4 Immediate Early Protein
  • Suitable for: WB, ELISA, ICC/IF
  • Reacts with: Species independent
  • Isotype: IgG1

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概述

  • 产品名称

    抗单纯疱疹病毒1 ICP4 Immediate Early蛋白抗体[10F1]
  • 描述

    小鼠单克隆抗体[10F1] to单纯疱疹病毒1 ICP4 Immediate Early蛋白
  • 宿主

    Mouse
  • 特异性

    ELISA titer 1/25,600 absorption of 0.10
  • 经测试应用

    适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性

    与反应: Species independent
  • 免疫原

    Tissue, cells or virus corresponding to HSV 1 ICP4 Immediate Early Protein.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.4
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    10F1
  • 骨髓瘤

    NS1/1-Ag4-1
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • double stranded DNA Virus
    • Herpes simplex

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab6514于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (3)
1/800.
ELISA
1/25600.
ICC/IF (2)
1/25600.
说明
WB
1/800.
ELISA
1/25600.
ICC/IF
1/25600.

靶标

  • 相关性

    This IE protein is a multifunctional protein capable of migrating to the nucleus, binding to DNA, trans-activating other viral genes, and autoregulating its own synthesis. Binding of ICP4 to alpha genes causes repression of their expression. ICP4 also acts as a transcriptional activator of beta and gamma genes.
  • 细胞定位

    Nuclear
  • 别名

    • Herpes simplex virus 1 ICP4 antibody
    • HSV RS1 antibody
    • ICP4 antibody
    • Immediate early protein antibody
    • RS1 antibody
    • Transcriptional regulator ICP4 antibody
    see all

图片

  • Western blot - Anti-HSV 1 ICP4 Immediate Early Protein  antibody [10F1] (ab6514)
    Western blot - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514)
    Lane 1 : Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/200 dilution
    Lane 2 : Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/400 dilution
    Lane 3 : Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/800 dilution
    Lane 4 : Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/1600 dilution

    All lanes : BCIP/NBT. HSV-1 MacIntyre/Vero infected cell extract

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Mouse secondary at 1/5000 dilution
  • Western blot - Anti-HSV 1 ICP4 Immediate Early Protein  antibody [10F1] (ab6514)
    Western blot - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514)This image is courtesy of an anonymous Abreview
    All lanes : Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/1000 dilution

    Lane 1 : HeLa cells (whole cell lysate) - mock infection, cytosolic fraction
    Lane 2 : HeLa cells (whole cell lysate) - mock infection, membrane fraction
    Lane 3 : HeLa cells (whole cell lysate) - mock infection, nuclear fraction
    Lane 4 : HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours - cytosolic fraction
    Lane 5 : HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours -membrane fraction
    Lane 6 : HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours - nuclear fraction
    Lane 7 : HeLa cells (whole cell lysate) - mock infected
    Lane 8 : HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP conjugated sheep anti-mouse IgG

    Observed band size: 97 kDa why is the actual band size different from the predicted?



    As shown, ICP4 is detected mainly in the nuclear fraction of infected cells (lane 6). The presence of ICP4 in the cytosolic fraction (lane 4) probably represents the protein undergoing synthesis.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-HSV 1 ICP4 Immediate Early Protein  antibody [10F1] (ab6514)
    Immunocytochemistry/ Immunofluorescence - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514)This image is courtesy of an anonymous Abreview
    ab6514 (1/5000) staining HSV1 ICP4 immediate Early Protein in mouse 293T cells transiently transfected with a plasmid expressing HSV ICP4 (green). Cells were formaldehyde-fixed and incubated with the primary antibody for 1 hour. A FITC conjugated rabbit polyclonal antibody was used as the secondary antibody.

    See Abreview

实验方案

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • Datasheet download

    Download

文献 (41)

发表研究结果有使用 ab6514?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab6514 被引用在 41 文献中.

  • Seyffert M  et al. The HSV-1 Transcription Factor ICP4 Confers Liquid-Like Properties to Viral Replication Compartments. Int J Mol Sci 22:N/A (2021). PubMed: 33923223
  • Sun B  et al. Regulation of host and virus genes by neuronal miR-138 favours herpes simplex virus 1 latency. Nat Microbiol 6:682-696 (2021). PubMed: 33558653
  • Murray-Nerger LA  et al. Lamin B1 acetylation slows the G1 to S cell cycle transition through inhibition of DNA repair. Nucleic Acids Res 49:2044-2064 (2021). PubMed: 33533922
  • Justice JL  et al. Systematic profiling of protein complex dynamics reveals DNA-PK phosphorylation of IFI16 en route to herpesvirus immunity. Sci Adv 7:N/A (2021). PubMed: 34144993
  • Abrahamson EE  et al. Modeling Aß42 Accumulation in Response to Herpes Simplex Virus 1 Infection: 2D or 3D? J Virol N/A:N/A (2020). PubMed: 33268524
View all Publications for this product

客户评价及客户问答

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1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

提交于 Feb 11 2009

Western blot abreview for Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
10 µg
Specification
HeLa cells
Treatment
Infected with HSV KOS for 16 hours
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

提交于 Sep 28 2007

Western blot abreview for Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (Vero Cells)
Loading amount
10 µg
Specification
Vero Cells
Treatment
Infected with HSV KOS
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

提交于 Aug 14 2007

Immunocytochemistry/ Immunofluorescence abreview for Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa adherent cells)
Specification
HeLa adherent cells
Fixative
Formaldehyde
Permeabilization
Yes - 0.5 % NP40
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Read More

Abcam user community

Verified customer

提交于 Jul 20 2007

Immunocytochemistry/ Immunofluorescence abreview for Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (293T cells, kidney epithelium)
Specification
293T cells, kidney epithelium
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

提交于 Jul 27 2006

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