Anti-HspBP1抗体(ab3858)
Key features and details
- Rabbit polyclonal to HspBP1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-HspBP1抗体 -
描述
兔多克隆抗体to HspBP1 -
宿主
Rabbit -
特异性
This antibody recognises HspBP1 (39kDa) in Western Blots. -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Fusion protein corresponding to Human HspBP1.
-
阳性对照
- This antibody gave a positive signal in HeLa cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39.3 kDa).
|
|
| ICC/IF |
Use a concentration of 5 µg/ml.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39.3 kDa). |
|
ICC/IF
Use a concentration of 5 µg/ml. |
靶标
-
功能
Inhibits HSPA1A chaperone activity by changing the conformation of the ATP-binding domain of HSPA1A and interfering with ATP binding. Interferes with ubiquitination mediated by STUB1 and inhibits chaperone-assisted degradation of immature CFTR. -
组织特异性
Ubiquitous. -
序列相似性
Contains 4 ARM repeats. - Information by UniProt
-
数据库链接
- Entrez Gene: 23640 Human
- Omim: 612939 Human
- SwissProt: Q9NZL4 Human
- Unigene: 53066 Human
-
别名
- 1500019G21Rik antibody
- FES1 antibody
- Heat shock protein 70 binding protein antibody
see all
图片
-
Immunocytochemistry/ Immunofluorescence - Anti-HspBP1 antibody (ab3858)
ICC/IF image of ab3858 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab3858, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in MCF7 cells.
-
Western blot - Anti-HspBP1 antibody (ab3858)Lane 1 : Anti-HspBP1 antibody (ab3858) at 1/500 dilution
Lane 2 : Anti-HspBP1 antibody (ab3858) at 1/1000 dilution
All lanes : MCF-7 Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39.3 kDa
Observed band size: 39.3 kDa
Exposure time: 2 minutesWestern blot using ab3858 on 20 ug per lane of MCF-7 Whole Cell Lysate. Secondary: Goat anti-rabbit IgG HRP conjugate ab6721 (1/2000). Exposure time: 2 mins.
Lane 1: ab3858 at 1/500.
Lane 2: ab3858 at 1/1000.
实验方案
数据表及文件
-
Datasheet download
文献 (0)
ab3858 尚未被引用在任何文献中。
