Key features and details
- Rabbit polyclonal to HspBP1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
特异性This antibody recognises HspBP1 (39kDa) in Western Blots.
经测试应用适用于: WB, ICC/IFmore details
Fusion protein corresponding to Human HspBP1.
- This antibody gave a positive signal in HeLa cells.
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab3858 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39.3 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
功能Inhibits HSPA1A chaperone activity by changing the conformation of the ATP-binding domain of HSPA1A and interfering with ATP binding. Interferes with ubiquitination mediated by STUB1 and inhibits chaperone-assisted degradation of immature CFTR.
序列相似性Contains 4 ARM repeats.
- Information by UniProt
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ICC/IF image of ab3858 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab3858, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in MCF7 cells.
Lane 1 : Anti-HspBP1 antibody (ab3858) at 1/500 dilution
Lane 2 : Anti-HspBP1 antibody (ab3858) at 1/1000 dilution
All lanes : MCF-7 Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39.3 kDa
Observed band size: 39.3 kDa
Exposure time: 2 minutes
Western blot using ab3858 on 20 ug per lane of MCF-7 Whole Cell Lysate. Secondary: Goat anti-rabbit IgG HRP conjugate ab6721 (1/2000). Exposure time: 2 mins.
Lane 1: ab3858 at 1/500.
Lane 2: ab3858 at 1/1000.