Application
Western blot
Sample
Human Cell lysate - whole cell (cell lysates in 25% Nupage LSD, 10% DTT, 65% milli)
Gel Running Conditions
Reduced Denaturing (12.5% Acrylamide gel)
Loading amount
35 µg
Specification
cell lysates in 25% Nupage LSD, 10% DTT, 65% milli
Blocking step
Rockland blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1:1 Rockland blocking buffer: PBS+0,1%T
Dilution
1/2
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: IRDye 800CW Goat Anti-Mouse
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Detection
Detection method
oddysey
Exposure
6 minute(s) and 0 second(s)
Bands
Specific: 70 kDa
Positive control
K-562
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 May 27 2015